Reversible histone acetylation involved in transcriptional regulation of WT1 gene

IMR OpenIR

	Reversible histone acetylation involved in transcriptional regulation of WT1 gene
其他题名	Reversible Histone Acetylation Involved in Transcriptional Regulation of WT1 Gene
	Shao Yangguang 1; Lu Jun 1; Cheng Cao 1; Cui Liguo 1; Zhang Guoping 1; Huang Baiqu 1
	2007
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	39 期号:12 页码:931-938
摘要	To validate the involvement of reversible histone acetylation in the transcriptional regulation of human Wilms' tumor 1 gene (WT1), we analyzed the roles of histone deacetylases (HDACs) and histone acetyltransferase in this epigenetic process. Of the six HDACs (HDAC1-6) examined, HDAC4 and HDAC5 were found to have significant repressing effects on the activity of the WT1 reporter gene, as revealed by luciferase reporter assays and quantitative real-time reverse transcription-polymerase chain reaction assays. Luciferase reporter assays showed that the histone acetyltransferase p300 was able to counteract the HDAC4/HDAC5-mediated repression and that p300/CBP synergized with transcription factors Sp1, c-Myb, and Ets-1 in activation of the WT1 reporter. Chromatin immunoprecipitation experiments showed that p300 promotes the acetylation level of histone H3 at the WT1 intronic enhancer. Based on these data, we proposed a hypothetical model for the involvement of reversible histone acetylation in transcriptional regulation of the WT1 gene. This study provides further insight into the mechanisms of transcriptional regulation of the WT1 gene and WT1-associated diseases treatment.
其他摘要	To validate the involvement of reversible histone acetylation in the transcriptional regulation of human Wilms' tumor 1 gene (WT1), we analyzed the roles of histone deacetylases (HDACs) and histone acetyltransferase in this epigenetic process. Of the six HDACs (HDAC1-6) examined, HDAC4 and HDAC5 were found to have significant repressing effects on the activity of the WT1 reporter gene, as revealed by luciferase reporter assays and quantitative real-time reverse transcription-polymerase chain reaction assays. Luciferase reporter assays showed that the histone acetyltransferase p300 was able to counteract the HDAC4/HDAC5-mediated repression and that p300/CBP synergized with transcription factors Sp1, c-Myb, and Ets-1 in activation of the WT1 reporter. Chromatin immunoprecipitation experiments showed that p300 promotes the acetylation level of histone H3 at the WT1 intronic enhancer. Based on these data, we proposed a hypothetical model for the involvement of reversible histone acetylation in transcriptional regulation of the WT1 gene. This study provides further insight into the mechanisms of transcriptional regulation of the WT1 gene and WT1-associated diseases treatment.
关键词	WILMS-TUMOR ACETYLTRANSFERASE P300 DEACETYLASE ENHANCER PROTEIN GATA-1 SP1 histone acetylation WT1 HDAC p300/CBP transcriptional regulation
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3088023
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151458
专题	中国科学院金属研究所
作者单位	1.上海市地震局 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Shao Yangguang,Lu Jun,Cheng Cao,et al. Reversible histone acetylation involved in transcriptional regulation of WT1 gene[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2007,39(12):931-938.
APA	Shao Yangguang,Lu Jun,Cheng Cao,Cui Liguo,Zhang Guoping,&Huang Baiqu.(2007).Reversible histone acetylation involved in transcriptional regulation of WT1 gene.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,39(12),931-938.
MLA	Shao Yangguang,et al."Reversible histone acetylation involved in transcriptional regulation of WT1 gene".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 39.12(2007):931-938.