Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)

IMR OpenIR

	Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)
其他题名	Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)
	Chen Bingru 1; Wang Chunyu 1; Wang Ping 1; Zhu Zhenxing 1; Xu Ning 2; Shi Guishan 2; Yu Miao 2; Wang Nai 2; Li Jihong 2; Hou Jiaming 2; Li Shujie 2; Zhou Yufei 1; Gao Shijie 2; Lu Xiaochun 1; Huang Ruidong 1
	2019
发表期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
卷号	18 期号:11 页码:2446-2456
摘要	Starch is the most important component in endosperm of sorghum grain. Usually, two types of starch are present: amylose (AM) and amylopectin (AP). The levels of AM and AP contents play a significant role in the appearance, structure, and quality of sorghum grains and in marketing applications. In the present study, a panel of 634 sorghum (Sorghum bicolor (L.) Moench) accessions were evaluated for starch, AM, and AP contents of grain, which included a mini core collection of 242 accessions from the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in India, and 252 landraces and 140 cultivars from China. The average starch content was 67.64% and the average AM and AP contents were 20.19 and 79.81%, respectively. We developed a total of 260 000 high-confidence single nucleotide polymorphism (SNP) markers in the panel of 634 accessions of S. bicolor using specific locus amplified fragment sequencing (SLAF-seq). We performed genome-wide association studies (GWAS) of starch, AM, and AM/AP of grain and SNP markers based on a mixed linear model (MLM). In total, 70 significant association signals were detected for starch, AM, and AM/AP ratio of grain with P<4.452x10(-7), of which 10 SNPs were identified with significant starch, 51 SNPs were associated with AM, and nine SNPs were associated with the AM/AP ratio. The Gene Ontology (GO) analysis identified 12 candidate genes at five QTLs associated with starch metabolism within the 200-kb intervals, located on chromosomes 1, 5, 6, and 9. Of these genes, Sobic.006G036500.1 encodes peptidyl-prolyl cis-trans-isomerase CYP38 responsible for hexose monophosphate shunt (HMS) and Sobic.009G071800 encodes 6-phospho-fructokinase (PFK), which is involved in the embden-meyerhof pathway (EMP). Kompetitive allele specific PCR (KASP) markers were developed to validate the GWAS results. The C allele is correlated with a high starch content, while the T allele is linked with a low level of starch content, and provides reliable haplotypes for MAS in sorghum quality improvement.
关键词	MAIZE KERNEL COMPOSITION QUANTITATIVE TRAIT LOCI GRAIN QUALITY TRAITS AGRONOMIC TRAITS RICE DIGESTIBILITY POLYMORPHISM EVOLUTION GENE sorghum genome-wide association mapping (GWAS) starch content amylose (AM) candidate genes KASP
收录类别	CSCD
语种	英语
资助项目	[earmarked fund for China Agriculture Research System]
CSCD记录号	CSCD:6614235
引用统计	被引频次：7[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151529
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Jilin Acad Agr Sci, Institute Crop Germplasm Resources, Gongzhuling 136100, Peoples R China
推荐引用方式 GB/T 7714	Chen Bingru,Wang Chunyu,Wang Ping,et al. Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(11):2446-2456.
APA	Chen Bingru.,Wang Chunyu.,Wang Ping.,Zhu Zhenxing.,Xu Ning.,...&Huang Ruidong.(2019).Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench).JOURNAL OF INTEGRATIVE AGRICULTURE,18(11),2446-2456.
MLA	Chen Bingru,et al."Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)".JOURNAL OF INTEGRATIVE AGRICULTURE 18.11(2019):2446-2456.