| Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench) | |
| Alternative Title | Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench) |
| Chen Bingru1; Wang Chunyu1; Wang Ping1; Zhu Zhenxing1; Xu Ning2; Shi Guishan2; Yu Miao2; Wang Nai2; Li Jihong2; Hou Jiaming2; Li Shujie2; Zhou Yufei1; Gao Shijie2; Lu Xiaochun1; Huang Ruidong1 | |
| 2019 | |
| Source Publication | JOURNAL OF INTEGRATIVE AGRICULTURE
 |
| ISSN | 2095-3119 |
| Volume | 18Issue:11Pages:2446-2456 |
| Abstract | Starch is the most important component in endosperm of sorghum grain. Usually, two types of starch are present: amylose (AM) and amylopectin (AP). The levels of AM and AP contents play a significant role in the appearance, structure, and quality of sorghum grains and in marketing applications. In the present study, a panel of 634 sorghum (Sorghum bicolor (L.) Moench) accessions were evaluated for starch, AM, and AP contents of grain, which included a mini core collection of 242 accessions from the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in India, and 252 landraces and 140 cultivars from China. The average starch content was 67.64% and the average AM and AP contents were 20.19 and 79.81%, respectively. We developed a total of 260 000 high-confidence single nucleotide polymorphism (SNP) markers in the panel of 634 accessions of S. bicolor using specific locus amplified fragment sequencing (SLAF-seq). We performed genome-wide association studies (GWAS) of starch, AM, and AM/AP of grain and SNP markers based on a mixed linear model (MLM). In total, 70 significant association signals were detected for starch, AM, and AM/AP ratio of grain with P<4.452x10(-7), of which 10 SNPs were identified with significant starch, 51 SNPs were associated with AM, and nine SNPs were associated with the AM/AP ratio. The Gene Ontology (GO) analysis identified 12 candidate genes at five QTLs associated with starch metabolism within the 200-kb intervals, located on chromosomes 1, 5, 6, and 9. Of these genes, Sobic.006G036500.1 encodes peptidyl-prolyl cis-trans-isomerase CYP38 responsible for hexose monophosphate shunt (HMS) and Sobic.009G071800 encodes 6-phospho-fructokinase (PFK), which is involved in the embden-meyerhof pathway (EMP). Kompetitive allele specific PCR (KASP) markers were developed to validate the GWAS results. The C allele is correlated with a high starch content, while the T allele is linked with a low level of starch content, and provides reliable haplotypes for MAS in sorghum quality improvement. |
| Keyword | MAIZE KERNEL COMPOSITION QUANTITATIVE TRAIT LOCI GRAIN QUALITY TRAITS AGRONOMIC TRAITS RICE DIGESTIBILITY POLYMORPHISM EVOLUTION GENE sorghum genome-wide association mapping (GWAS) starch content amylose (AM) candidate genes KASP |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [earmarked fund for China Agriculture Research System] |
| CSCD ID | CSCD:6614235 |
| Citation statistics |
Cited Times:1[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/151529 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.Jilin Acad Agr Sci, Institute Crop Germplasm Resources, Gongzhuling 136100, Peoples R China |
| Recommended Citation GB/T 7714 | Chen Bingru,Wang Chunyu,Wang Ping,et al. Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2019,18(11):2446-2456. |
| APA | Chen Bingru.,Wang Chunyu.,Wang Ping.,Zhu Zhenxing.,Xu Ning.,...&Huang Ruidong.(2019).Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench).JOURNAL OF INTEGRATIVE AGRICULTURE,18(11),2446-2456. |
| MLA | Chen Bingru,et al."Genome-wide association study for starch content and constitution in sorghum (Sorghum bicolor (L.) Moench)".JOURNAL OF INTEGRATIVE AGRICULTURE 18.11(2019):2446-2456. |
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