Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation

IMR OpenIR

	Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation
其他题名	Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation
	Zhang Zhi 1; Yu Bo 1; Tao Guizhou 2
	2009
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
卷号	122 期号:19 页码:2360-2365
摘要	Background Apoptosis is a major cause of ischemic heart dysfunction. Apelin, the endogenous ligand for the G-protein-coupled AN receptor, has been reported to exert cardioprotective effects during myocardial injury. The aim of this study was to investigate the effects of apelin on apoptosis of rat cardiomyocytes induced by glucose deprivation (GD) and study the related signaling pathway.
其他摘要	Background Apoptosis is a major cause of ischemic heart dysfunction. Apelin, the endogenous ligand for the G-protein-coupled APJ receptor, has been reported to exert cardioprotective effects during myocardial injury. The aim of this study was to investigate the effects of apelin on apoptosis of rat cardiomyocytes induced by glucose deprivation (GD) and study the related signaling pathway.Methods Apelin and APJ mRNA expression were determined by RT-PCR in neonatal rat cardiomyocytes during different durations of GD. Cardiomyocyte apoptosis was detected by annexin V-FITC/propidium iodide (PI) staining after GD for 12 hours with or without apelin-13 (10 and 100 nmol/L) pretreatment. Protein levels of Akt and the mammalian target of rapamycin (mTOR) as well as cell apoptosis were detected in the presence or absence of LY294002 (a phosphatidylinositol 3-kinases (PI3K) inhibitor) or rapamycin (a mTOR inhibitor).Results Apelin mRNA expression was up-regulated when cardiomyocytes were exposed to GD for 6, 12, 18, and 24 hours compared with the base level (P >0.05, P <0.01, P <0.01, P <0.01). However, when cardiomyocytes were exposed to GD for up to 36 hours, apelin mRNA expression was 17% lower than the base level (P <0.05). APJ mRNA expression paralleled that of apelin. Apelin-13 pretreatment at 100 nmol/L significantly inhibited GD-induced cardiomyocyte apoptosis (P <0.05) and increased Akt and mTOR phosphorylation (P <0.01, P <0.01). At the same time apelin-13 (100 nmol/L) up-regulated Bcl-2 protein expression and down-regulated Bax and cleaved caspase-3 expression (P <0.01, P <0.05, P <0.05). The anti-apoptotic effect of apelin-13 was blocked by LY294002 (P <0.01) but not by rapamycin. Conclusions The endogenous apelin-APJ system is compensatorily up-regulated and ultimately down-regulated following sustained myocardial ischemia. Apelin protects against ischemic cardiomyocyte apoptosis via activation of the PI3K/Akt pathway.
关键词	ISCHEMIC-HEART FAILURE REPERFUSION INJURY APJ RECEPTOR CONTRACTILITY LOCALIZATION EXPRESSION AUTOPHAGY SURVIVAL SALVAGE SYSTEM apelin apoptosis cardiomyocyte phosphatidylinositol 3-OH kinase (PI3K)/Akt
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3681779
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151908
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Liaoning Med University, Affiliated Hosp 1, Dept Cardiol, Jinzhou 121001, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Zhang Zhi,Yu Bo,Tao Guizhou. Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation[J]. CHINESE MEDICAL JOURNAL,2009,122(19):2360-2365.
APA	Zhang Zhi,Yu Bo,&Tao Guizhou.(2009).Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation.CHINESE MEDICAL JOURNAL,122(19),2360-2365.
MLA	Zhang Zhi,et al."Apelin protects against cardiomyocyte apoptosis induced by glucose deprivation".CHINESE MEDICAL JOURNAL 122.19(2009):2360-2365.