| The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells | |
| Alternative Title | The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells |
| Wang Yong1; Yue Dan2; Li Kai1; Liu YiLi1; Ren ChangShan1; Wang Ping1 | |
| 2010 | |
| Source Publication | ASIAN JOURNAL OF ANDROLOGY
 |
| ISSN | 1008-682X |
| Volume | 12Issue:6Pages:841-852 |
| Abstract | Hepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important in promoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels in the HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were used to investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca2+ imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycle progression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 and PC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdown suppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca2+ entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G2/M phase and suppressed HGF-induced cell proliferation. Collectively, our results indicate that TRPC6 has an important role in HGF-induced DU145 and PC3 cell proliferation. |
| Other Abstract | Hepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important in promoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels in the HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were used to investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca 2+ imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycle progression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 and PC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdown suppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca 2+ entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G 2 |
| Keyword | HEPATOCYTE GROWTH-FACTOR EPITHELIAL-CELLS C-MET CHANNELS EXPRESSION CALCIUM CA2+ RECEPTOR BENIGN GENE hepatocyte growth factor human prostate cancer proliferation TRPC6 |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:4073832 |
| Citation statistics |
Cited Times:1[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/151982 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.Tianjin Med University, Coll Med Lab Sci, Tianjin 300070, Peoples R China |
| Recommended Citation GB/T 7714 | Wang Yong,Yue Dan,Li Kai,et al. The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(6):841-852. |
| APA | Wang Yong,Yue Dan,Li Kai,Liu YiLi,Ren ChangShan,&Wang Ping.(2010).The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells.ASIAN JOURNAL OF ANDROLOGY,12(6),841-852. |
| MLA | Wang Yong,et al."The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells".ASIAN JOURNAL OF ANDROLOGY 12.6(2010):841-852. |
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