The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells

IMR OpenIR

	The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells
其他题名	The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells
	Wang Yong 1; Yue Dan 2; Li Kai 1; Liu YiLi 1; Ren ChangShan 1; Wang Ping 1
	2010
发表期刊	ASIAN JOURNAL OF ANDROLOGY
ISSN	1008-682X
卷号	12 期号:6 页码:841-852
摘要	Hepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important in promoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels in the HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were used to investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca2+ imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycle progression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 and PC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdown suppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca2+ entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G2/M phase and suppressed HGF-induced cell proliferation. Collectively, our results indicate that TRPC6 has an important role in HGF-induced DU145 and PC3 cell proliferation.
其他摘要	Hepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important in promoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels in the HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were used to investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca 2+ imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycle progression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 and PC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdown suppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca 2+ entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G 2
关键词	HEPATOCYTE GROWTH-FACTOR EPITHELIAL-CELLS C-MET CHANNELS EXPRESSION CALCIUM CA2+ RECEPTOR BENIGN GENE hepatocyte growth factor human prostate cancer proliferation TRPC6
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:4073832
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/151985
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Tianjin Med University, Coll Med Lab Sci, Tianjin 300070, Peoples R China
推荐引用方式 GB/T 7714	Wang Yong,Yue Dan,Li Kai,et al. The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(6):841-852.
APA	Wang Yong,Yue Dan,Li Kai,Liu YiLi,Ren ChangShan,&Wang Ping.(2010).The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells.ASIAN JOURNAL OF ANDROLOGY,12(6),841-852.
MLA	Wang Yong,et al."The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells".ASIAN JOURNAL OF ANDROLOGY 12.6(2010):841-852.