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The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells
Alternative TitleThe role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells
Wang Yong1; Yue Dan2; Li Kai1; Liu YiLi1; Ren ChangShan1; Wang Ping1
2010
Source PublicationASIAN JOURNAL OF ANDROLOGY
ISSN1008-682X
Volume12Issue:6Pages:841-852
AbstractHepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important in promoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels in the HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were used to investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca2+ imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycle progression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 and PC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdown suppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca2+ entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G2/M phase and suppressed HGF-induced cell proliferation. Collectively, our results indicate that TRPC6 has an important role in HGF-induced DU145 and PC3 cell proliferation.
Other AbstractHepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important in promoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels in the HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were used to investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca 2+ imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycle progression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 and PC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdown suppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca 2+ entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G 2
KeywordHEPATOCYTE GROWTH-FACTOR EPITHELIAL-CELLS C-MET CHANNELS EXPRESSION CALCIUM CA2+ RECEPTOR BENIGN GENE hepatocyte growth factor human prostate cancer proliferation TRPC6
Indexed ByCSCD
Language英语
CSCD IDCSCD:4073832
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/151985
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.Tianjin Med University, Coll Med Lab Sci, Tianjin 300070, Peoples R China
Recommended Citation
GB/T 7714
Wang Yong,Yue Dan,Li Kai,et al. The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells[J]. ASIAN JOURNAL OF ANDROLOGY,2010,12(6):841-852.
APA Wang Yong,Yue Dan,Li Kai,Liu YiLi,Ren ChangShan,&Wang Ping.(2010).The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells.ASIAN JOURNAL OF ANDROLOGY,12(6),841-852.
MLA Wang Yong,et al."The role of TRPC6 in HGF-induced cell proliferation of human prostate cancer DU145 and PC3 cells".ASIAN JOURNAL OF ANDROLOGY 12.6(2010):841-852.
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