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Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells
Alternative TitleEffect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells
Li XiaoDong; Zhang XinRui; Li ZhiHao; Yang Yang; Zhang Duo; Zheng Heng; Dong ShuYing; Chen Juan; Zeng XianDong
2017
Source PublicationCHINESE MEDICAL JOURNAL
ISSN0366-6999
Volume130Issue:6Pages:717-721
AbstractBackground: Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP-13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis.
Other AbstractBackground: Dendritic cells are professional antigen-presenting cells found in an immature state in epithelia and interstitial space, where they capture antigens such as pathogens or damaged tissue. Matrix metallopeptidase 13 (MMP- 13), a member of the collagenase subfamily, is involved in many different cellular processes and is expressed in murine bone marrow-derived dendritic cells (DCs). The function of MMP-13 in DCs is not well understood. Here, we investigated the effect of MMP-13 on DC maturation, apoptosis, and phagocytosis. Methods: Bone marrow-derived dendritic cells were obtained fiom C57BL/6 mice. One short-interfering RNA specific for MMP- 13 was used to transfect DCs. MMP-13-silenced DCs and control DCs were prepared, and apoptosis was measured using real-time polymerase chain reaction and Western blotting. MMP-13-silenced DCs and control DCs were analyzed for surface expression of CD80 and CD86 and phagocytosis capability using flow cytometry. Results: Compared to the control DCs, MMP- 13-silenced DCs increased expression of anti-apoptosis-related genes, BAGl (control group vs. MMP-13-silenced group: 4.08 ± 0.60 vs. 6.11 ± 0.87, P = 0.008), BCL-2 (control group vs. MMP-13-silenced group: 7.54 ± 0.76 vs. 9.54 ± 1.29, P = 0.036), and TP73 (control group vs. MMP- 13-silenced group: 4.33 ± 0.29 vs. 5.60 ±0.32, P = 0.001 ) and decreased apoptosis-related genes, CASPI (control group vs. MMP- 13-silenced group: 3.79±0.67 vs. 2.54±0.39, P - 0.019), LTBR (control group vs. MMP- 13-silenced group: 9.23 ±1.25 vs. 6.24 ± 1.15, P = 0.012), and CASP4 (control group vs. MMP-13-silenced group: 2.07 ± 0.56 vs. 0.35 ±0.35, P = 0.002). Protein levels confirmed the same expression pattern. MMP- 13-silenced groups decreased expression of CD86 on DCs; however, there was no statistical difference in CD80 surface expression. Furthermore, MMP-13-silenced groups exhibited weaker phagocytosis capability. Conclusion: These results indicate that MMP-13 inhibition dampens DC maturation, apoptosis, and phagocytosis.
KeywordMETALLOPROTEINASES MODULATORS EXPRESSION MMP-13 Apoptosis Dendritic Cell Matrix Metallopeptidase 13 Maturation Phagocytosis
Indexed ByCSCD
Language英语
Funding Project[Natural Science Foundation of Liaoning Province]
CSCD IDCSCD:5932850
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/152191
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Li XiaoDong,Zhang XinRui,Li ZhiHao,et al. Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells[J]. CHINESE MEDICAL JOURNAL,2017,130(6):717-721.
APA Li XiaoDong.,Zhang XinRui.,Li ZhiHao.,Yang Yang.,Zhang Duo.,...&Zeng XianDong.(2017).Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells.CHINESE MEDICAL JOURNAL,130(6),717-721.
MLA Li XiaoDong,et al."Effect of Matrix Metallopeptidase 13 on the Function of Mouse Bone Marrow-derived Dendritic Cells".CHINESE MEDICAL JOURNAL 130.6(2017):717-721.
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