Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato

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	Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
其他题名	Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
	Lu Tao; Shi Jiewei; Sun Zhouping; Qi Mingfang; Liu Yufeng; Li Tianlai
	2017
发表期刊	JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
ISSN	1673-1581
卷号	18 期号:7 页码:635-648
摘要	Objective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings ("Liaoyuanduoli", n=160) were divided into four parts: Part 1, served as control under 25 degrees C, 500 mu mol/(m(2).s); Part 2, spayed with distilled water (H2O) under 35 degrees C, 1000 mu mol/(m(2).s) (HH); Part 3, spayed with 100 mu mol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 mu mol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F-v/F-m), and increased the excitation energy distribution coefficient of PSII (beta); DCMU and MV aggravated the partition imbalance of the excitation energy (gamma) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETRI and Y-I) significantly decreased whereas acceptor and donor side limitation of PSI (Y-NA and Y-ND) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy Y-NPQ and Y-NO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.
其他摘要	Objective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings (“Liaoyuanduoli”, n=160) were divided into four parts: Part 1, served as control under 25 ℃, 500 μmol/(m~2·s); Part 2, spayed with distilled water (H_2O) under 35 ℃, 1000 μmol/(m~2·s) (HH); Part 3, spayed with 100 μmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 μmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F_v/F_m), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETR_I and Y_I) significantly decreased whereas acceptor and donor side limitation of PSI (Y_(NA) and Y_(ND)) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy YNPQ and YNO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity.
关键词	CHLOROPLAST NAD(P)H DEHYDROGENASE PHOTOSYSTEM-I CHLOROPHYLL FLUORESCENCE SUBLETHAL CONCENTRATIONS PHYSIOLOGICAL-ROLE PROTON GRADIENT HEAT-STRESS DELTA-PSI FLOW PHOTOSYNTHESIS Photoinhibition Photoprotection Thylakoid membrane Tomato
收录类别	CSCD
语种	英语
资助项目	[China Agriculture Research System] ; [National Natural Science Foundation of China] ; [National Key Technologies R & D Program of China]
CSCD记录号	CSCD:6034514
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/152308
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Lu Tao,Shi Jiewei,Sun Zhouping,et al. Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato[J]. JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,2017,18(7):635-648.
APA	Lu Tao,Shi Jiewei,Sun Zhouping,Qi Mingfang,Liu Yufeng,&Li Tianlai.(2017).Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,18(7),635-648.
MLA	Lu Tao,et al."Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 18.7(2017):635-648.