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Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq
Alternative TitleComparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq
Wang Shouming; Li Weijia; Liu Yuexue; Li He; Ma Yue; Zhang Zhihong
2017
Source PublicationJOURNAL OF INTEGRATIVE AGRICULTURE
ISSN2095-3119
Volume16Issue:4Pages:828-844
AbstractGenes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate (EMS)-mutagenized lines of Fragaria vesca accession Yellow Wonder (YW), two fruit shapes are observed: wild-type long fruit and mutated shortened fruit (s.f). In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin (GA)-deficient mutant, and four complementary DNA (cDNA) libraries separately constructed from flower buds and small green fruits of YW and sf were sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YW1 and sfl, and 28 differentially expressed GA pathway genes were identified between YW2 and sf2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR (qRT-PCR), and the results were highly concordant with the RNA-Seq results. This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.
Other AbstractGenes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate (EMS)-mutagenized lines of Fragaria vesca accession Yellow Wonder (YW), two fruit shapes are observed: wild-type long fruit and mutated shortened fruit (sf). In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin (GA)-deficient mutant, and four complementary DNA (cDNA) libraries separately constructed from flower buds and small green fruits of YW and sf were sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YW1 and sf1, and 28 differentially expressed GA pathway genes were identified between YW2 and sf 2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR (qRT-PCR), and the results were highly concordant with the RNA-Seq results. This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.
KeywordDE-NOVO CHARACTERIZATION GIBBERELLIN BIOSYNTHESIS GREEN-REVOLUTION DEFICIENT MUTANT CELL-DIVISION TOMATO MUTANT GENE ARABIDOPSIS PROTEIN GENOME strawberry fruit shape transcriptome RNA-Seq differentially expressed gene
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China]
CSCD IDCSCD:5962859
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/152646
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang Shouming,Li Weijia,Liu Yuexue,et al. Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2017,16(4):828-844.
APA Wang Shouming,Li Weijia,Liu Yuexue,Li He,Ma Yue,&Zhang Zhihong.(2017).Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq.JOURNAL OF INTEGRATIVE AGRICULTURE,16(4),828-844.
MLA Wang Shouming,et al."Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq".JOURNAL OF INTEGRATIVE AGRICULTURE 16.4(2017):828-844.
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