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Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
Alternative TitleQuantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
Wang Weiping1; Chai Ali2; Shi Yanxia2; Xie Xuewen2; Li Baoju2
2015
Source PublicationSPECTROSCOPY AND SPECTRAL ANALYSIS
ISSN1000-0593
Volume35Issue:5Pages:1243-1247
AbstractClubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P. brassicae are laborious, time-consuming and low sensitivity. Rapid and effective detection methods are needed. The objective of this study is to develop a Fourier transform infrared spectrometer (FTIR) technique for detection of P. brassicae effectively and accurately. FTIR and Real-time PCR techniques were applied in quantitative detection of P. brassicae. Chinese cabbages were inoculated with P. brassicae. By analyzing the FTIR spectra of P. brassicae, infected clubroots and healthy roots, three specific bands 1 105, 1 145 and 1 228 cm(-1) were selected. According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value, quantitative evaluation model of P. brassicae was established based on FTIR y=34. 17+12. 24x(1)-9. 81x(2)-6. 05x(3), r=0. 98 (p<0. 05). To validate accuracy of the model, 10 clubroot samples were selected randomly from field, and detected by FTIR spectrum model, the results showed that the average error is 1. 60%. This demonstrated that the FTIR technology is an available one for the quantitative detection of P. brassicae in clubroot, and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot.
KeywordREAL-TIME PCR PLASMODIOPHORA-BRASSICAE CRUCIFEROUS CROPS RAPID DETECTION DISEASE Fourier transform infrared spectroscopy FTIR Chinese cabbage clubroot Plasmodiophora brassicae Model
Indexed ByCSCD
Language英语
CSCD IDCSCD:5410956
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/152743
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.Chinese Acad Agr Sci, Institute Vegetables & Flowers, Beijing 100081, Peoples R China Institute Vegetables & Flowers
Recommended Citation
GB/T 7714
Wang Weiping,Chai Ali,Shi Yanxia,et al. Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2015,35(5):1243-1247.
APA Wang Weiping,Chai Ali,Shi Yanxia,Xie Xuewen,&Li Baoju.(2015).Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,35(5),1243-1247.
MLA Wang Weiping,et al."Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 35.5(2015):1243-1247.
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