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Effects of Er3+ on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro
Alternative TitleEffects of Er 3+ on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro
Zhang Jinchao1; Sun Jing1; Zhang Dawei2; Li Yaping1; Hao Xiaohong1; Qin Xinying1
2011
Source PublicationJOURNAL OF RARE EARTHS
ISSN1002-0721
Volume29Issue:5Pages:507-510
AbstractA series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) test, alkaline phosphatase (ALP) activity measurement, oil red O stain and measurement, mineralized function and quantitive real time RT-PCR (qRT-PCR) were employed to assess the effects of Er3+ on the proliferation, differentiation and mineralization function of primary osteoblasts (OBs) in vitro at cell and molecular levels. The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1x10(-7) mol/L, but had no effect at other concentrations. Er3+ inhibited the differentiation of OBs at concentrations of 1x10(-8), 1x10(-7), and 1x10(-6) mol/L, but had no effect at a higher concentration of 1x10(-5) mol/L. Er3+ had no effect on the transdifferentiation of OBs at tested concentrations. Er3+ inhibited the mineralization function of OBs at concentrations of 1x10(-7), 1x10(-6), and 1x10(-5) mol/L, but had no effect at a lower concentration of 1x10(-8) mol/L. The expression of the mRNA for runt-related transcription factor 2 (RUNX-2) and peroxisome proliferators activated receptor gamma (PPAR-gamma) was down-regulated in the presence of 1x10(-6) mol/L Er3+. These findings suggested that Er3+ might have negative effect on bone metabolism.
Other AbstractA series of experimental methods including 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT)test,alkaline phosphatase (ALP)activity measurement,oil red O stain and measurement,mineralized function and quantitive real time RT-PCR (qRT-PCR)were employed to assess the effects of Er3+ on the proliferation,differentiation and mineralization function of primary osteoblasts (OBs)in vitro at cell and molecular levels.The results indicated that Er3+ inhibited the proliferation of OBs at a concentration of 1 x 10~(-7)mol/L,but had no effect at other concentrations.Er3 inhibited the differentiation of OBs at concentrations of 1 x 10~(-8),1 x 10~(-7),and 1 x 10~(-6)mol/L,but had no effect at a higher concentration of 1 x 10~(-5)mol/L.Er3+ had no effect on the transdifferentiation of OBs at tested concentrations.Er3+ inhibited the mineralization function of OBs at concentrations of x 10~(-7),1 x 10~(-6),and 1 x 10~(-5)mol/L,but had no effect at a lower concentration of 1 x 10~(-8)mol/L.The expression of the mRNA for runt-related transcription factor 2 (RUNX-2)and peroxisome proliferators activated receptor y (PPAR-y)was down-regulated in the presence of 1 x 10~(-6)mol/L Er3+.These findings suggested that Er3+ might have negative effect on bone metabolism.
KeywordCELLS PROTEIN GROWTH erbium ion osteoblasts proliferation differentiation mineralization rare earths
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Foundation for Key Program of Ministry of Education of China] ; [Returned Scholars of Hebei Province] ; [Nature Science Key Foundation of Hebei Province] ; [Natural Science Foundation of Hebei University]
CSCD IDCSCD:4381795
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/152916
Collection中国科学院金属研究所
Affiliation1.河北大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Zhang Jinchao,Sun Jing,Zhang Dawei,et al. Effects of Er3+ on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro[J]. JOURNAL OF RARE EARTHS,2011,29(5):507-510.
APA Zhang Jinchao,Sun Jing,Zhang Dawei,Li Yaping,Hao Xiaohong,&Qin Xinying.(2011).Effects of Er3+ on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro.JOURNAL OF RARE EARTHS,29(5),507-510.
MLA Zhang Jinchao,et al."Effects of Er3+ on the proliferation, differentiation and mineralization function of primary mouse osteoblasts in vitro".JOURNAL OF RARE EARTHS 29.5(2011):507-510.
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