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Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress
Alternative TitlePuerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress ★
Wu Yingying1; Xue Bing2; Li Xiaojin1; Liu Hongchen1
2012
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume7Issue:33Pages:2583-2591
AbstractOxidative stress may be the unifying factor for the injury caused by hyperglycemia in diabetic peripheral neuropathy. Puerarin is the major isoflavonoid derived from Radix puerariae and has been shown to be effective in increasing superoxide dismutase activity. This study sought to investigate the neuroprotective effect of puerarin on high glucose-induced oxidative stress and Schwann cell apoptosis in vitro. Intracellular reactive oxygen radicals and mitochondrial transmembrane potential were detected by flow cytometry analysis. Apoptosis was confirmed by TUNEL and oxidative stress was monitored using an enzyme-linked immunosorbent assay for the DNA marker 8-hydroxy-2-deoxyguanosine. The expression levels of bax and bcl-2 were analyzed by quantitative real-time reverse transcriptase-PCR, while protein expression of cleaved caspase-3 and -9 were analyzed by means cif western blotting. Results suggested that puerarin treatment inhibited high glucose-induced oxidative stress, mitochondrial depolarization and apoptosis in a dose-dependent manner. Furthermore, puerarin treatment downregulated Bax expression, upregulated bcl-2 expression and attenuated the activation of caspase-3 and -9. Overall, our results indicated that puerarin antagonized high glucose-induced oxidative stress and apoptosis in Schwann cells.
Other AbstractOxidative stress may be the unifying factor for the injury caused by hyperglycemia in diabetic peripheral neuropathy. Puerarin is the major isoflavonoid derived from Radix puerariae and has been shown to be effective in increasing superoxide dismutase activity. This study sought to investigate the neuroprotective effect of puerarin on high glucose-induced oxidative stress and Schwann cell apoptosis in vitro . Intracellular reactive oxygen radicals and mitochondrial transmembrane potential were detected by flow cytometry analysis. Apoptosis was confirmed by TUNEL and oxidative stress was monitored using an enzyme-linked immunosorbent assay for the DNA marker 8-hydroxy-2-deoxyguanosine. The expression levels of bax and bcl-2 were analyzed by quantitative real-time reverse transcriptase-PCR, while protein expression of cleaved caspase-3 and -9 were analyzed by means of western blotting. Results suggested that puerarin treatment inhibited high glucose-induced oxidative stress, mitochondrial depolarization and apoptosis in a dose-dependent manner. Furthermore, puerarin treatment downregulated Bax expression, upregulated bcl-2 expression and attenuated the activation of caspase-3 and -9. Overall, our results indicated that puerarin antagonized high glucose-induced oxidative stress and apoptosis in Schwann cells.
KeywordSALVIANOLIC ACID B DIABETIC-NEUROPATHY ENDOTHELIAL-CELLS POLYOL PATHWAY GROWTH-FACTOR IN-VITRO RAT PROLIFERATION COMPLICATIONS ACTIVATION puerarin diabetic peripheral neuropathy hyperglycemia Schwann cell apoptosis caspase mitochondrial transmembrane potential oxidative stress 8-hydroxy-2-deoxyguanosine reactive oxygen radical
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China]
CSCD IDCSCD:4694391
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/153045
Collection中国科学院金属研究所
Affiliation1.中国人民解放军总医院
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Wu Yingying,Xue Bing,Li Xiaojin,et al. Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress[J]. NEURAL REGENERATION RESEARCH,2012,7(33):2583-2591.
APA Wu Yingying,Xue Bing,Li Xiaojin,&Liu Hongchen.(2012).Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress.NEURAL REGENERATION RESEARCH,7(33),2583-2591.
MLA Wu Yingying,et al."Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress".NEURAL REGENERATION RESEARCH 7.33(2012):2583-2591.
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