Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress

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	Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress
其他题名	Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress ★
	Wu Yingying 1; Xue Bing 2; Li Xiaojin 1; Liu Hongchen 1
	2012
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	7 期号:33 页码:2583-2591
摘要	Oxidative stress may be the unifying factor for the injury caused by hyperglycemia in diabetic peripheral neuropathy. Puerarin is the major isoflavonoid derived from Radix puerariae and has been shown to be effective in increasing superoxide dismutase activity. This study sought to investigate the neuroprotective effect of puerarin on high glucose-induced oxidative stress and Schwann cell apoptosis in vitro. Intracellular reactive oxygen radicals and mitochondrial transmembrane potential were detected by flow cytometry analysis. Apoptosis was confirmed by TUNEL and oxidative stress was monitored using an enzyme-linked immunosorbent assay for the DNA marker 8-hydroxy-2-deoxyguanosine. The expression levels of bax and bcl-2 were analyzed by quantitative real-time reverse transcriptase-PCR, while protein expression of cleaved caspase-3 and -9 were analyzed by means cif western blotting. Results suggested that puerarin treatment inhibited high glucose-induced oxidative stress, mitochondrial depolarization and apoptosis in a dose-dependent manner. Furthermore, puerarin treatment downregulated Bax expression, upregulated bcl-2 expression and attenuated the activation of caspase-3 and -9. Overall, our results indicated that puerarin antagonized high glucose-induced oxidative stress and apoptosis in Schwann cells.
其他摘要	Oxidative stress may be the unifying factor for the injury caused by hyperglycemia in diabetic peripheral neuropathy. Puerarin is the major isoflavonoid derived from Radix puerariae and has been shown to be effective in increasing superoxide dismutase activity. This study sought to investigate the neuroprotective effect of puerarin on high glucose-induced oxidative stress and Schwann cell apoptosis in vitro . Intracellular reactive oxygen radicals and mitochondrial transmembrane potential were detected by flow cytometry analysis. Apoptosis was confirmed by TUNEL and oxidative stress was monitored using an enzyme-linked immunosorbent assay for the DNA marker 8-hydroxy-2-deoxyguanosine. The expression levels of bax and bcl-2 were analyzed by quantitative real-time reverse transcriptase-PCR, while protein expression of cleaved caspase-3 and -9 were analyzed by means of western blotting. Results suggested that puerarin treatment inhibited high glucose-induced oxidative stress, mitochondrial depolarization and apoptosis in a dose-dependent manner. Furthermore, puerarin treatment downregulated Bax expression, upregulated bcl-2 expression and attenuated the activation of caspase-3 and -9. Overall, our results indicated that puerarin antagonized high glucose-induced oxidative stress and apoptosis in Schwann cells.
关键词	SALVIANOLIC ACID B DIABETIC-NEUROPATHY ENDOTHELIAL-CELLS POLYOL PATHWAY GROWTH-FACTOR IN-VITRO RAT PROLIFERATION COMPLICATIONS ACTIVATION puerarin diabetic peripheral neuropathy hyperglycemia Schwann cell apoptosis caspase mitochondrial transmembrane potential oxidative stress 8-hydroxy-2-deoxyguanosine reactive oxygen radical
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:4694391
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/153045
专题	中国科学院金属研究所
作者单位	1.中国人民解放军总医院 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Wu Yingying,Xue Bing,Li Xiaojin,et al. Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress[J]. NEURAL REGENERATION RESEARCH,2012,7(33):2583-2591.
APA	Wu Yingying,Xue Bing,Li Xiaojin,&Liu Hongchen.(2012).Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress.NEURAL REGENERATION RESEARCH,7(33),2583-2591.
MLA	Wu Yingying,et al."Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress".NEURAL REGENERATION RESEARCH 7.33(2012):2583-2591.