Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells

IMR OpenIR

	Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells
其他题名	Effects of Genistein on Proliferation and Cell Cycle of Salivary Adenoid Cystic Carcinoma Cells
	Ma Jie 1; Wang Jie 2; Zhong Ming 2; Wang Zhaoyuan 2
	2007
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
卷号	19 期号:1 页码:64-67
摘要	Objective: To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC- 83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods: Effects of genistein on the growth of SACC- 83 cells in vitro were measured with MTT assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdk1 proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSS11.5 software. Results: Genistein inhibited the cell proliferation in a dose- dependant and time- dependant manner. The genistein- treated SACC- 83 cells were arrested in the G2/ M phase and had lower contents of CyclinB1 and Cdk1 proteins compared with the control group. Conclusion: The growth inhibiting effect of genistein on SACC- 83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle.
其他摘要	Objective: To investigate the growth inhibiting effect of tyrosine protein kinase inhibitor, genistein, on human salivary adenoid cystic carcinoma SACC-83 cell line in vitro, and its effects on the expression of CyclinB1 protein and cell cycle. Methods: Effects of genistein on the growth of SACC-83 cells in vitro were measured with MTT assay. Cell cycle was detected with flow cytometry. The expressions of CyclinB1 and Cdk1 proteins were measured with Western blot method, and the results of protein expression were quantitatively analyzed by FluorChem V2.0 software. The results were statistically analyzed by SPSS11.5 software. Results: Genistein inhibited the cell proliferation in a dose-dependant and time-dependant manner. The genistein-treated SACC-83 cells were arrested in the G2/M phase and had lower contents of CyclinB1 and Cdk1 proteins compared with the control group. Conclusion: The growth inhibiting effect of genistein on SACC-83 cells may be associated with the regulations of genistein on the CyclinB1 and Cdk1 protein expressions and the cell cycle.
关键词	PROSTATE-CANCER CELLS APOPTOSIS INDUCTION GROWTH adenoid cystic carcinoma genistein CyclinB1 cell cycle
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:2920371
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/153283
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Ma Jie,Wang Jie,Zhong Ming,et al. Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2007,19(1):64-67.
APA	Ma Jie,Wang Jie,Zhong Ming,&Wang Zhaoyuan.(2007).Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells.CHINESE JOURNAL OF CANCER RESEARCH,19(1),64-67.
MLA	Ma Jie,et al."Effects of genistein on proliferation and cell cycle of salivary adenoid cystic carcinoma cells".CHINESE JOURNAL OF CANCER RESEARCH 19.1(2007):64-67.