Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model

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	Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model
其他题名	Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model
	Jiang Yuanjun; Gong Daxin; Liu Haibo; Yang Chunming; Sun Zhixi; Kong Chuize
	2008
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
卷号	29 期号:6 页码:713-719
摘要	Aim: The present study was conducted to investigate whether alpha-lipoic acid (alpha-LA) is able to reverse impaired bladder function induced by diabetes in a rat model and to explore the possible mechanism mediating the effect. Methods: Male Sprague-Dawley rats were divided randomly into 3 age-matched groups: control, diabetes mellitus (DM) treated with vehicle, and DM with alpha-LA treatment. The diabetic rats were induced by a single intraperitoneal (ip) injection of streptozotocin (60 mg/kg). Six weeks after the induction of DM, the two groups received another 6 weeks of treatment with vehicle or alpha-LA (100 mg/kg, ip). Body weight and blood glucose levels were measured weekly. The bladder function was evaluated by in vitro cystometry. The oxidative stress status was determined by biochemical methods, and the level of nerve growth factor was investigated by enzyme-linked immunosorbent assay. Results: The streptozotocin-induced diabetic rats showed impaired bladder function characterized by increased bladder capacity, decreased bladder contractility (voiding efficiency), and an increase in residual urine. Treatment with alpha-LA significantly normalized the increased bladder capacity for inducing voiding, single-voided volume, and post-void residual volume. alpha-LA treatment significantly reversed the increased level of malondialdehyde and reduced the activities of both superoxide dismutase and catalase. DM caused a decrease in the bladder nerve growth factor (NGF) level, and alpha-LA upregulated the level of NGF in the diabetic rat bladder. Conclusion: These results indicate that alpha-LA has a beneficial effect on diabetes-induced cystopathy by ameliorating oxidative stress and normalizing the NGF level in the bladder.
其他摘要	Aim: The present study was conducted to investigate whether alpha-lipoic acid (α-LA) is able to reverse impaired bladder function induced by diabetes in a rat model and to explore the possible mechanism mediating the effect. Methods: Male Sprague_Dawley rats were divided randomly into 3 age-matched groups: control, diabetes mellitus (DM) treated with vehicle, and DM with α-LA treatment. The diabetic rats were induced by a single intraperitoneal (ip) injection of streptozotocin (60 mg/kg). Six weeks after the induction of DM, the two groups received another 6 weeks of treatment with vehicle or α-LA (100 mg/kg, ip). Body weight and blood glucose levels were measured weekly. The bladder function was evaluated by in vitro cystometry. The oxidative stress status was determined by biochemical methods, and the level of nerve growth factor was investigated by enzyme-linked immunosorbent assay. Results: The streptozotocin-induced diabetic rats showed impaired bladder function characterized by increased bladder capacity, decreased bladder contractility (voiding efficiency), and an increase in residual urine. Treatment with α-LA significantly normalized the increased bladder capacity for inducing voiding, single-voided volume, and post-void residual volume. α-LA treatment significantly reversed the increased level of malondialdehyde and reduced the activities of both superoxide dismutase and catalase. DM caused a decrease in the bladder nerve growth factor (NGF) level, and α-LA upregulated the level of NGF in the diabetic rat bladder. Conclusion: These results indicate that α-LA has a beneficial effect on diabetes-induced cystopathy by ameliorating oxidative stress and normalizing the NGF level in the bladder.
关键词	NERVE-GROWTH-FACTOR OXIDATIVE STRESS BLADDER DYSFUNCTION LIPID-PEROXIDATION URINARY-BLADDER COMPLICATIONS EXPRESSION MECHANISM THERAPY POLYNEUROPATHY alpha-lipoic acid diabetic cystopathy diabetes mellitus neuropathy bladder oxidative stress nerve growth factor rats
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3301358
引用统计	被引频次：2[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/153310
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Jiang Yuanjun,Gong Daxin,Liu Haibo,et al. Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model[J]. ACTA PHARMACOLOGICA SINICA,2008,29(6):713-719.
APA	Jiang Yuanjun,Gong Daxin,Liu Haibo,Yang Chunming,Sun Zhixi,&Kong Chuize.(2008).Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model.ACTA PHARMACOLOGICA SINICA,29(6),713-719.
MLA	Jiang Yuanjun,et al."Ability of alpha-lipoic acid to reverse the diabetic cystopathy in a rat model".ACTA PHARMACOLOGICA SINICA 29.6(2008):713-719.