Synthesis of novel hydrophobic media and purification of recombinant HBsAg

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	Synthesis of novel hydrophobic media and purification of recombinant HBsAg
Alternative Title	Synthesis of novel hydrophobic media and purification of recombinant HBsAg
	Wang Y 1; Wang Q 1; Xiao NQ 1; Liu DW 1; Ye SD 1; Luo X 1; Guan GF 1; Wei ZL 1; Chen WG 1; Bai F 1
	1999
Source Publication	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
Volume	15 Issue:1 Pages:39-46
Abstract	A novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r-HBsAg). r-HBsAg was further purified to the final products by following a downstream procedure(1). The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r-HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r-HBsAg products have reached the same level as other similar kinds of products.
Keyword	Chinese hamster ovary (CHO) recombinant hepatitis B surface antigen (r-HBsAg) hydrophobic interaction chromatography (HIC) reverse phase haemagglutination assay (RPHA)
Indexed By	CSCD
Language	英语
CSCD ID	CSCD:496710
Citation statistics	Cited Times:1[CSCD] [CSCD Record]
Document Type	期刊论文
Identifier	http://ir.imr.ac.cn/handle/321006/153338
Collection	中国科学院金属研究所
Affiliation	1.中山大学 2.北京大学 3.中国科学院金属研究所
Recommended Citation GB/T 7714	Wang Y,Wang Q,Xiao NQ,et al. Synthesis of novel hydrophobic media and purification of recombinant HBsAg[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,1999,15(1):39-46.
APA	Wang Y.,Wang Q.,Xiao NQ.,Liu DW.,Ye SD.,...&Bai F.(1999).Synthesis of novel hydrophobic media and purification of recombinant HBsAg.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,15(1),39-46.
MLA	Wang Y,et al."Synthesis of novel hydrophobic media and purification of recombinant HBsAg".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 15.1(1999):39-46.

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