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Synthesis of novel hydrophobic media and purification of recombinant HBsAg
Alternative TitleSynthesis of novel hydrophobic media and purification of recombinant HBsAg
Wang Y1; Wang Q1; Xiao NQ1; Liu DW1; Ye SD1; Luo X1; Guan GF1; Wei ZL1; Chen WG1; Bai F1
1999
Source PublicationCHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN1005-9040
Volume15Issue:1Pages:39-46
AbstractA novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r-HBsAg). r-HBsAg was further purified to the final products by following a downstream procedure(1). The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r-HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r-HBsAg products have reached the same level as other similar kinds of products.
KeywordChinese hamster ovary (CHO) recombinant hepatitis B surface antigen (r-HBsAg) hydrophobic interaction chromatography (HIC) reverse phase haemagglutination assay (RPHA)
Indexed ByCSCD
Language英语
CSCD IDCSCD:496710
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/153341
Collection中国科学院金属研究所
Affiliation1.中山大学
2.北京大学
3.中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang Y,Wang Q,Xiao NQ,et al. Synthesis of novel hydrophobic media and purification of recombinant HBsAg[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,1999,15(1):39-46.
APA Wang Y.,Wang Q.,Xiao NQ.,Liu DW.,Ye SD.,...&Bai F.(1999).Synthesis of novel hydrophobic media and purification of recombinant HBsAg.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,15(1),39-46.
MLA Wang Y,et al."Synthesis of novel hydrophobic media and purification of recombinant HBsAg".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 15.1(1999):39-46.
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