Synthesis of novel hydrophobic media and purification of recombinant HBsAg

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	Synthesis of novel hydrophobic media and purification of recombinant HBsAg
其他题名	Synthesis of novel hydrophobic media and purification of recombinant HBsAg
	Wang Y 1; Wang Q 1; Xiao NQ 1; Liu DW 1; Ye SD 1; Luo X 1; Guan GF 1; Wei ZL 1; Chen WG 1; Bai F 1
	1999
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	15 期号:1 页码:39-46
摘要	A novel hydrophobic medium with propyl as functional group and Sepharose 6B as matrix was designed and synthesized. The comparison of the hydrophobic medium synthesized with the commercial products was made by hydrophobic interaction chromatography(HIC) in isolating recombinant hepatitis B surface antigen(r-HBsAg). r-HBsAg was further purified to the final products by following a downstream procedure(1). The results indicate that the synthesized hydrophobic medium possesses a stable structure and desired physical and chemical properties. They were used to purify r-HBsAg with a high yield and purity. Both the immunity and stability of hepatitis vaccine made by the r-HBsAg products have reached the same level as other similar kinds of products.
关键词	Chinese hamster ovary (CHO) recombinant hepatitis B surface antigen (r-HBsAg) hydrophobic interaction chromatography (HIC) reverse phase haemagglutination assay (RPHA)
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:496710
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/153341
专题	中国科学院金属研究所
作者单位	1.中山大学 2.北京大学 3.中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang Y,Wang Q,Xiao NQ,et al. Synthesis of novel hydrophobic media and purification of recombinant HBsAg[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,1999,15(1):39-46.
APA	Wang Y.,Wang Q.,Xiao NQ.,Liu DW.,Ye SD.,...&Bai F.(1999).Synthesis of novel hydrophobic media and purification of recombinant HBsAg.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,15(1),39-46.
MLA	Wang Y,et al."Synthesis of novel hydrophobic media and purification of recombinant HBsAg".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 15.1(1999):39-46.