DNA barcoding provides distinction between Radix Astragali and its adulterants

IMR OpenIR

	DNA barcoding provides distinction between Radix Astragali and its adulterants
其他题名	DNA barcoding provides distinction between Radix Astragali and its adulterants
	Guo HaiYan 1; Wang WeiWei 1; Yang Ning 1; Guo BaoLin 3; Zhang Sun 1; Yang RuiJing 1; Yuan Ye 1; Yu JunLin 4; Hu SongNian 1; Sun QiShi 2; Yu Jun 1
	2010
发表期刊	SCIENCE CHINA-LIFE SCIENCES
ISSN	1674-7305
卷号	53 期号:8 页码:992-999
摘要	Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including China, Japan, and Korea. However, in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs.
其他摘要	Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species,DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A.membranaceus var.mongholica are commonly used as Radix Astragali in several Asian countries, including China, Japan, and Korea. However,in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore,a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study,we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, mat K, rbc L, and cox I sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5%and 14.1%, respectively, whereas mat K was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the mat K sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and mat K are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs
关键词	5S-RIBOSOMAL-RNA SPACER DOMAIN GENETIC DIVERSITY RAPD ANALYSIS IN-VITRO AUTHENTICATION IDENTIFICATION MEMBRANACEUS SEQUENCE HUANGQI MARKERS Radix Astragali DNA barcode Astragalus adulterant identification
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [National High-Tech Research and Development Program of China]
CSCD记录号	CSCD:4053450
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/153364
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院金属研究所 3.Chinese Acad Med Sci, Peking Union Med Coll, Institute Med Plant Dev, Beijing 100094, Peoples R China Peking Union Med Coll Institute Med Plant Dev 4.Tonghua Normal Coll, Dept Chemistry, Tonghua 134002, Peoples R China
推荐引用方式 GB/T 7714	Guo HaiYan,Wang WeiWei,Yang Ning,et al. DNA barcoding provides distinction between Radix Astragali and its adulterants[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(8):992-999.
APA	Guo HaiYan.,Wang WeiWei.,Yang Ning.,Guo BaoLin.,Zhang Sun.,...&Yu Jun.(2010).DNA barcoding provides distinction between Radix Astragali and its adulterants.SCIENCE CHINA-LIFE SCIENCES,53(8),992-999.
MLA	Guo HaiYan,et al."DNA barcoding provides distinction between Radix Astragali and its adulterants".SCIENCE CHINA-LIFE SCIENCES 53.8(2010):992-999.