| DNA barcoding provides distinction between Radix Astragali and its adulterants | |
| Alternative Title | DNA barcoding provides distinction between Radix Astragali and its adulterants |
| Guo HaiYan1; Wang WeiWei1; Yang Ning1; Guo BaoLin3; Zhang Sun1; Yang RuiJing1; Yuan Ye1; Yu JunLin4; Hu SongNian1; Sun QiShi2; Yu Jun1 | |
| 2010 | |
| Source Publication | SCIENCE CHINA-LIFE SCIENCES
 |
| ISSN | 1674-7305 |
| Volume | 53Issue:8Pages:992-999 |
| Abstract | Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species, DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A. membranaceus var. mongholica are commonly used as Radix Astragali in several Asian countries, including China, Japan, and Korea. However, in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore, a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study, we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, matK, rbcL, and coxI sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5% and 14.1%, respectively, whereas matK was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the matK sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and matK are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs. |
| Other Abstract | Based on variable nuclear and/or organellar DNA sequences among vastly divergent species as well as morphologically indistinguishable species,DNA barcoding is widely applicable in species identification, biodiversity studies, forensic analyses, and authentication of medicinal plants. The roots of Astragalus membranaceus and A.membranaceus var.mongholica are commonly used as Radix Astragali in several Asian countries, including China, Japan, and Korea. However,in addition to the two species recorded in the Chinese Pharmacopoeia, there are twenty-three species from different genera including Astragalus, Oxytropis, Hedysarum, and Glycyrrhiza, which have been used as adulterants not only in trading markets but also by the herbal medicine industry. Therefore,a simple, reliable, and accurate classification method is important for distinguishing authentic Radix Astragali from its adulterants. In this study,we acquired data for 37 samples from four related genera within the family Fabaceae. Then we compared four candidate DNA barcoding markers using ITS, mat K, rbc L, and cox I sequences from nuclear, chloroplast, and mitochondrial genomes, all commonly used for plants to identify genetic variations among genera, intraspecies, and interspecies. We observed higher divergences among genera and interspecies for ITS, which have the average Kimura 2-parameter distances of 4.5%and 14.1%, respectively, whereas mat K was found to have sufficient divergence at the intraspecific level. Moreover, two indels detected in the mat K sequence are useful for PCR studies in distinguishing Radix Astragali from its adulterants. This study suggests that the combined barcoding regions of ITS and mat K are superior barcodes for Radix Astragali and further studies should focus on evaluating the applicability and accuracy of such combined markers for a wide range of traditional Chinese herbs |
| Keyword | 5S-RIBOSOMAL-RNA SPACER DOMAIN GENETIC DIVERSITY RAPD ANALYSIS IN-VITRO AUTHENTICATION IDENTIFICATION MEMBRANACEUS SEQUENCE HUANGQI MARKERS Radix Astragali DNA barcode Astragalus adulterant identification |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National Natural Science Foundation of China] ; [National High-Tech Research and Development Program of China] |
| CSCD ID | CSCD:4053450 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/153364 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院 2.中国科学院金属研究所 3.Chinese Acad Med Sci, Peking Union Med Coll, Institute Med Plant Dev, Beijing 100094, Peoples R China Peking Union Med Coll Institute Med Plant Dev 4.Tonghua Normal Coll, Dept Chemistry, Tonghua 134002, Peoples R China |
| Recommended Citation GB/T 7714 | Guo HaiYan,Wang WeiWei,Yang Ning,et al. DNA barcoding provides distinction between Radix Astragali and its adulterants[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(8):992-999. |
| APA | Guo HaiYan.,Wang WeiWei.,Yang Ning.,Guo BaoLin.,Zhang Sun.,...&Yu Jun.(2010).DNA barcoding provides distinction between Radix Astragali and its adulterants.SCIENCE CHINA-LIFE SCIENCES,53(8),992-999. |
| MLA | Guo HaiYan,et al."DNA barcoding provides distinction between Radix Astragali and its adulterants".SCIENCE CHINA-LIFE SCIENCES 53.8(2010):992-999. |
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