Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system

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	Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system
其他题名	Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system ★
	Li Zhaoyang; Du Bing; Li Shengyang; Lv Xiangchuan; Zhou Shenglai; Yu Yang; Wang Wei; Zheng Zhihong
	2013
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	8 期号:2 页码:156-U97
摘要	Apolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.
其他摘要	Apolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.
关键词	LIPOPROTEIN-LIPASE TRANSCRIPTION FACTORS GENETIC ASSOCIATION EXPRESSION JUN DEFICIENCY ACTIVATION NEURONS DISEASE METABOLISM neural regeneration basic research apolipoprotein C2 promoter dual-luciferase reporter assay transcriptional activity regulatory elements grants-supported paper neuroregeneration
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Education Department of Liaoning Province]
CSCD记录号	CSCD:4753407
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/153729
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Li Zhaoyang,Du Bing,Li Shengyang,et al. Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system[J]. NEURAL REGENERATION RESEARCH,2013,8(2):156-U97.
APA	Li Zhaoyang.,Du Bing.,Li Shengyang.,Lv Xiangchuan.,Zhou Shenglai.,...&Zheng Zhihong.(2013).Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system.NEURAL REGENERATION RESEARCH,8(2),156-U97.
MLA	Li Zhaoyang,et al."Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system".NEURAL REGENERATION RESEARCH 8.2(2013):156-U97.