|Cyclophilin A affects Bcl-2 and Bax expression following beta-amyloid fragment 25-35-induced injury to PC12 cells|
|Alternative Title||Cyclophilin A affects Bcl-2 and Bax expression following beta-amyloid fragment 25-35-induced injury to PC12 cells
|Cheng Li; Zhang Chaodong
|Source Publication||NEURAL REGENERATION RESEARCH
|Abstract||BACKGROUND: Cyclophilin A can protect neurons against oxidative stress.|
|Other Abstract||BACKGROUND: Cyclophilin A can protect neurons against oxidative stress.OBJECTIVE: To investigate the effect of cyclophilin A on Bcl-2 and Bax protein expression in pheochro-mocytoma (PC12) cells treated with beta-amyloid fragment 25-35 (Aβ_(25-35)), and to verify the protection pathway of cyclophilin A. DESIGN, TIME AND SETTING: The initial experiment was performed at the Laboratory of Department of Neurology, First Clinical College, China Medical University from November 2006 to July 2007.MATERIALS: PC12 cells were cultured at the Cell Center of Peking Union Medical College. Aβ_(25-35) (Sigma, USA), antibodies of Bcl-2 and Bax (Wuhan Boster, China), and recombinant human cyclophilin A (Biomol, USA) were used in this study. METHODS: PC12 cells were divided into three groups. Cells in the control group were incubated in culture medium. Cells in the Aβ_(25-35) injury group were incubated in medium containing a final concentration of 10 μmol/L of Aβ_(25-35). Cells in the cyclophilin A group were incubated in medium containing a final con-centration of 10 nmol/L of cyclophilin A for 30 minutes, and then treated with 10 μmol/L Aβ_(25-35).MAIN OUTCOME MEASURES: After 24 hours of culture, immunohistochemistry was used to detect Bcl-2 and Bax expression in PC12 cells. Annexin-V flow cytometry was employed to measure the apoptosis rate of PC12 cells. The MTT method was applied to examine the survival rate of PC12 cells. RESULTS: Bcl-2 expression decreased, whereas Bax expression increased in PC12 cells treated with Aβ_(25-35) (t = 2.277, 5.957, P < 0.05). However, in PC12 cells treated with Aβ_(25-35) and cyclophilin A, Bcl-2 expression increased and Bax expression decreased (t = 4.497, 2.531, P < 0.05). The survival rate of PC12 cells significantly decreased and the apoptosis rate increased (t=8.509, 22.886, P < 0.05) following Aβ_(25-35) treatment. Cyclophilin A enhanced the survival rate of PC12 cells to Aβ_(25-35)-induced apoptosis (t = 4.895, 10.042, P < 0.05). CONCLUSION: Cyclophilin A can increase Bcl-2 expression and decrease Bax expression in PC12 cells treated with Aβ_(25-35), which indicates that cyclophilin A has a protective effect on Aβ_(25-35)-induced injury to PC12 cells.|
pheochromocytoma (PC12) cells
beta-amyloid fragment 25-35
Cheng Li,Zhang Chaodong. Cyclophilin A affects Bcl-2 and Bax expression following beta-amyloid fragment 25-35-induced injury to PC12 cells[J]. NEURAL REGENERATION RESEARCH,2008,3(6):585-588.
Cheng Li,&Zhang Chaodong.(2008).Cyclophilin A affects Bcl-2 and Bax expression following beta-amyloid fragment 25-35-induced injury to PC12 cells.NEURAL REGENERATION RESEARCH,3(6),585-588.
Cheng Li,et al."Cyclophilin A affects Bcl-2 and Bax expression following beta-amyloid fragment 25-35-induced injury to PC12 cells".NEURAL REGENERATION RESEARCH 3.6(2008):585-588.
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