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Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells
Alternative TitleOverexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells
Yu Xiuling; Gao Dianwen
2013
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume8Issue:23Pages:2198-2203
AbstractA plasmid for cytoglobin expression, pAcGFP1-C1-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-1 alpha in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible factor-1 alpha expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increasing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-1 alpha are strongly up-regulated by hypoxia, and that there is a strong relationship between hypoxia-inducible factor-1 alpha and cytoglobin during hypoxic injury.
Other AbstractA plasmid for cytoglobin expression, pAcGFP1-C1-cytoglobin, was transfected into SH-SY5Y cells. Cobalt chloride was used to establish a model of hypoxia. Western blotting indicated that cytoglobin was overexpressed and there was low expression of hypoxia-inducible factor-1α in SH-SY5Y cells after transfection. Following cobalt chloride-induced hypoxia, cytoglobin and hypoxia-inducible factor-1α expression gradually increased in SH-SY5Y cells. Flow cytometry showed that with increasing duration of hypoxia, the proportion of normal cells significantly diminished in the transfected and non-transfected groups. The proportion of cells in the early stages of apoptosis increased. However, the proportion of apoptotic cells was significantly lower in the transfected group compared with the non-transfected group. These results demonstrate that cytoglobin and hypoxia-inducible factor-1α are strongly up-regulated by hypoxia, and that there is a strong relationship between hypoxia-inducible factor-1α and cytoglobin during hypoxic injury.
KeywordINDUCIBLE FACTOR-1 NEUROGLOBIN EXPRESSION BRAIN TISSUES ANOXIA DEATH neural regeneration cytoglobin hypoxia green fluorescent protein transfection SH-SY5Y cells cobalt chloride recombinant plasmid neuroprotection neuroregeneration
Indexed ByCSCD
Language英语
CSCD IDCSCD:4916341
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/154098
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Yu Xiuling,Gao Dianwen. Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells[J]. NEURAL REGENERATION RESEARCH,2013,8(23):2198-2203.
APA Yu Xiuling,&Gao Dianwen.(2013).Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells.NEURAL REGENERATION RESEARCH,8(23),2198-2203.
MLA Yu Xiuling,et al."Overexpression of cytoglobin gene inhibits hypoxic injury to SH-SY5Y neuroblastoma cells".NEURAL REGENERATION RESEARCH 8.23(2013):2198-2203.
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