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Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30
Alternative TitleEffect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30
Liang Chunyan; Ma Ping
2008
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume20Issue:2Pages:100-104
AbstractObjective: To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30. Methods: Chemically synthesized double stranded RNA( dsRNA) targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamine(TM)2000. The transfection efficiency was observed under a fluorescence confocal microscope. Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemical analysis. The effects on apoptosis of ZR-75-30 cells were assessed by FCAS. Results: The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30. The inhibition rate was 53.66% at mRNA level and 58.32% at protein level. After 24h, (8.36 +/- 0.20)% cells transfected with siRNA were induced to apoptosis. Conclusion: Chemically synthesized short Livin-siRNA can effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30. Livin RNAi has a potential value in gene therapy of breast cancer.
Other AbstractObjective:To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30.Methods:Chemically synthesized double stranded RNA(dsRNA)targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamine~(TM)2000.The transfection efficiency was observed under a fluorescence confocal microscope.Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemical:analysis.The effects on apoptosis of ZR-75-30 cells were assessed by FCAS.Results:The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30.The inhibition rate was 53.66% at mRNA level and 58.32% at protein level.After 24h,(8.36±0.20)%cells transfected with siRNA were induced to apoptosis.Conclusion:Chemically synthesized short Livin-siRNA can effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30.Livin RNAi has a potential value in gene therapy of breast cancer.
KeywordPROTEIN FAMILY MESSENGER-RNA INHIBITOR INTERFERENCE SURVIVIN SIRNA EXPRESSION MEMBER DEATH Livin breast cancer RNA interference apoptosis
Indexed ByCSCD
Language英语
CSCD IDCSCD:3299649
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/154108
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Liang Chunyan,Ma Ping. Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(2):100-104.
APA Liang Chunyan,&Ma Ping.(2008).Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30.CHINESE JOURNAL OF CANCER RESEARCH,20(2),100-104.
MLA Liang Chunyan,et al."Effect of RNAi-mediated gene silencing of Livin on apoptosis of human breast cancer cell line ZR-75-30".CHINESE JOURNAL OF CANCER RESEARCH 20.2(2008):100-104.
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