Association of inflammatory response gene polymorphism with atherothrombotic stroke in Northern Han Chinese

IMR OpenIR

	Association of inflammatory response gene polymorphism with atherothrombotic stroke in Northern Han Chinese
其他题名	Association of inflammatory response gene polymorphism with atherothrombotic stroke in Northern Han Chinese
	Zhao Jiuhan 1; Wang Xiaohong 1; Xu Jialiang 1; Li Nan 2; Shang Xiuli 1; He Zhiyi 1; Yang Jun 1
	2012
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	1672-9145
卷号	44 期号:12 页码:1023-1030
摘要	Atherosclerosis is an important pathophysiological basis of atherothrombotic stroke (ATS), and inflammation plays a significant role in atherosclerosis formation. In this study, single-nucleotide polymorphisms (SNPs) in three key inflammation-related genes, 5-lipoxygenase activating protein (ALOX5AP), phosphodiesterase 4D (PDE4D), and interleukin-1 (IL-1), were investigated to determine their association with ATS in Northern Han Chinese. Six-hundred and eighty-two ATS patients and 598 unrelated controls were recruited. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism and matrix-assisted laser desorption ionization time-of-flight mass spectrometry primer extension. The genotype and allele frequencies of each SNP were statistically analyzed. Risk of ATS was found for the ALOX5AP SG13S114A/T AA genotype (P 0.040) and A allele (P 0.033), PDE4D SNP83C/T TT genotype (P 0.010) and T allele (P 0.008) and SNP219A/G GG genotype (P 0.025) and G allele (P 0.022), and the IL-1-889C/T T allele (P 0.035). The differences still remained significant after adjustment. The ALOX5AP HapA haplotype was not correlated with ATS (P 0.834), but GCGA represented an at-risk haplotype (P 0.008). Furthermore, the PDE4D AA haplotype at SNP219-220 might be an at-risk haplotype (P 0.013), while GA might be a protective haplotype (P 0.005). The ALOX5AP (SG13S114A/T), PDE4D (SNP83C/T, 219A/G), and IL-1 (-889C/T) SNPs were associated with an increased risk of ATS in Northern Han Chinese.
其他摘要	Atherosclerosis is an important pathophysiological basis of atherothrombotic stroke (ATS), and inflammation plays a significant role in atherosclerosis formation. In this study, single-nucleotide polymorphisms (SNPs) in three key inflammation-related genes, 5-lipoxygenase activating protein (ALOX5AP), phosphodiesterase 4D (PDE4D), and interleu-kin-la (IL-la), were investigated to determine their association with ATS in Northern Han Chinese. Six-hundred and eighty-two ATS patients and 598 unrelated controls were recruited. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism and matrix-assisted laser desorption ionization time-of-flight mass spectrometry primer extension. The genotype and allele frequencies of each SNP were statistically analyzed. Risk of ATS was found for the ALOX5AP SG13S114A/T AA genotype (P = 0.040) and A allele (P = 0.033), PDE4D SNP83C/T TT genotype (P = 0.010) and T allele (P= 0.008) and SNP219A/G GG genotype (P = 0.025) and G allele (P = 0.022), and the IL-1a-889C/T T allele (P = 0.035). The differences still remained significant after adjustment. The ALOX5AP HapA haplotype was not correlated with ATS (P = 0.834), but GCGA represented an at-risk haplotype (P = 0.008). Furthermore, the PDE4D AA haplotype at SNP219-220 might be an at-risk haplotype (P = 0.013), while GA might be a protective haplotype (P = 0.005). The ALOX5AP (SG13S114A/T), PDE4D (SNP83C/T, 219A/G), and IL-la (-889C/T) SNPs were associated with an increased risk of ATS in Northern Han Chinese.
关键词	5-LIPOXYGENASE ACTIVATING PROTEIN CAROTID-ARTERY ATHEROSCLEROSIS ISCHEMIC-STROKE PHOSPHODIESTERASE 4D MYOCARDIAL-INFARCTION CEREBRAL INFARCTION ALOX5AP GENE PDE4D GENE POPULATION RISK inflammation polymorphism atherothrombotic stroke ALOX5AP PDE4D
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:4708879
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/154237
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.Jinzhou Cent Hosp, Department Neurol, Jinzhou 121000, Peoples R China
推荐引用方式 GB/T 7714	Zhao Jiuhan,Wang Xiaohong,Xu Jialiang,et al. Association of inflammatory response gene polymorphism with atherothrombotic stroke in Northern Han Chinese[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2012,44(12):1023-1030.
APA	Zhao Jiuhan.,Wang Xiaohong.,Xu Jialiang.,Li Nan.,Shang Xiuli.,...&Yang Jun.(2012).Association of inflammatory response gene polymorphism with atherothrombotic stroke in Northern Han Chinese.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,44(12),1023-1030.
MLA	Zhao Jiuhan,et al."Association of inflammatory response gene polymorphism with atherothrombotic stroke in Northern Han Chinese".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 44.12(2012):1023-1030.