Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB

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	Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB
其他题名	Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB
	Shen WenJing; Dai DongQiu; Teng Yue; Liu HongBo
	2008
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	14 期号:4 页码:595-600
摘要	AIM: To investigate the changes of methylation state and expression of RASSF1A gene in human gastric cancer cell lines SGC7901 and BGC823 which were treated in vitro with demethlylating agent 5-Aza-CdR in combination with histone deacetylase inhibitor NaB.
其他摘要	AIM: To investigate the changes of methylation state and expression of RASSF1A gene in human gastric cancer cell lines SGC7901 and BGC823 which were treated in vitro with demethlylating agent 5-Aza-CdR in combination with histone deacetylase inhibitor NaB.METHODS: After SGC7901 and BGC823 cells were treated with 5-Aza-CdR and/or NaB, the methylation state of RASSF1A gene was detected by methylation-specific PCR, and the changes in expression of mRNA and protein level of RASSF1A gene were observed by RT-PCR and Western-blotting before and after drug treatment.RESULTS: Hypermethylation was detected in the promoter region of RASSF1A gene in both SGC7901 and BGC823 cells, and there was no expression of this gene at both mRNA and protein level. After treatment with 5-Aza-CdR, demethylation occurred in the promoter region of RASSF1A gene, which subsequently induced re-expression of this gene. The treatment with NaB alone showed no effect on the methylation state and expression of RASSF1A gene. The combined treatment of 5-Aza-CdR and NaB induced complete demethylation of RASSF1A gene, leading to a significantly higher re-expression of the mRNA and protein of RASSF1A than those treated with 5-Aza-CdR alone (P < 0.05).CONCLUSION: Hypermethylation in the promoter region is related to inactivation of RASSF1A gene in human gastric cancer cell lines SGC7901 and BGC823, while demethlylating agent 5-Aza-CdR can reverse the methylation state of RASSF1A gene and induce itsre-expression. Histone deacetylase inhibitor NaB had a synergistic effect with 5-Aza-CdR in both demethylation and gene transcriptional regulation.(c) 2008 WJG. All rights reserved.
关键词	DNA METHYLTRANSFERASE INHIBITORS HISTONE DEACETYLASE INHIBITION CYCLE PROGRESSION METHYLATION 5-AZA-2'-DEOXYCYTIDINE EXPRESSION TUMOR HYPERMETHYLATION ACETYLATION CARCINOGENESIS 5-Aza-CdR NaB RASSF1A gene gastric cancer DNA methylation
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3317134
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/154534
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Shen WenJing,Dai DongQiu,Teng Yue,et al. Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(4):595-600.
APA	Shen WenJing,Dai DongQiu,Teng Yue,&Liu HongBo.(2008).Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB.WORLD JOURNAL OF GASTROENTEROLOGY,14(4),595-600.
MLA	Shen WenJing,et al."Regulation of demethylation and re-expression of RASSF1A gene in gastric cancer cell lines by combined treatment of 5-Aza-CdR and NaB".WORLD JOURNAL OF GASTROENTEROLOGY 14.4(2008):595-600.