Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays

IMR OpenIR

	Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays
其他题名	Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays
	Wang Yan 1; Sun GuoXiang 1; Jin Yang 4; Xie XiuMan 1; Liu YingChun 1; Ma DiDi 1; Zhang Jing 1; Gao JiaYue 1; Li YanFei 1
	2017
发表期刊	CHINESE JOURNAL OF NATURAL MEDICINES
ISSN	2095-6975
卷号	15 期号:4 页码:310-320
摘要	The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets (SHT), a widely used and commercially available herbal preparation, where high performance liquid chromatography (HPLC) with a diode array detector (DAD) was employed to obtain the fingerprint profiles. A simple linear quantitative fingerprint method (SLQFM) coupled with multi-ingredient simultaneous determination was developed to evaluate the quality consistency of the tested samples qualitatively and quantitatively. Additionally, the component-activity relationship between chromatographic fingerprints and total radical- scavenging capacity in vitro (as assessed using the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay) was investigated by partial least squares regression (PLSR) analysis to predict the antioxidant capacity of new samples from the chromatographic fingerprints and identify the main active constituents that can be used as the target markers for the quality control of SHT. In conclusion, the strategy developed in the present study was effective and reliable, which can be employed for holistic evaluation and accurate discrimination for the quality consistency of SHT preparations and other traditional Chinese medicine (TCM) and herbal preparations as well.
其他摘要	The present study was designed to establish a multi-wavelength quantitative fingerprinting method for San-Huang Tablets(SHT), a widely used and commercially available herbal preparation, where high performance liquid chromatography (HPLC) with a diode array detector (DAD) was employed to obtain the fingerprint profiles. A simple linear quantitative fingerprint method (SLQFM):oupled with multi-ingredient simultaneous determination was developed to evaluate the quality consistency of the tested samples qualitatively and quantitatively. Additionally, the component-activity relationship between chromatographic fingerprints and total radi-:al-scavenging capacity in vitro (as assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay) was investigated by partial least squares regression (PLSR) analysis to predict the antioxidant capacity of new samples from the chromatographic fingerprints and identify the main active constituents that can be used as the target markers for the quality control of SHT. In conclusion, the strategy developed in the present study was effective and reliable, which can be employed for holistic evaluation and accurate discrimination for the quality consistency of SHT preparations and other traditional Chinese medicine (TCM) and herbal preparations as well.
关键词	QUALITY CONSISTENCY EVALUATION HPLC FINGERPRINT SANHUANG TABLET CHROMATOGRAPHIC FINGERPRINTS ANTIOXIDANT ACTIVITY MEDICINE CHEMOMETRICS INGREDIENTS INJECTION EXTRACTS San-Huang Tablets Radical-scavenging activity Simple linear quantitative fingerprint method Quality evaluation
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Doctoral Research Fund of Dali University]
CSCD记录号	CSCD:5988712
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/154557
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.大理学院 3.中国科学院南京地理与湖泊研究所 4.Tradit Chinese Med Hosp Dali, Department Pharm, Dali 671000, Peoples R China Department Pharm
推荐引用方式 GB/T 7714	Wang Yan,Sun GuoXiang,Jin Yang,et al. Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2017,15(4):310-320.
APA	Wang Yan.,Sun GuoXiang.,Jin Yang.,Xie XiuMan.,Liu YingChun.,...&Li YanFei.(2017).Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays.CHINESE JOURNAL OF NATURAL MEDICINES,15(4),310-320.
MLA	Wang Yan,et al."Holistic evaluation of San-Huang Tablets using a combination of multi-wavelength quantitative fingerprinting and radical-scavenging assays".CHINESE JOURNAL OF NATURAL MEDICINES 15.4(2017):310-320.