Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification

IMR OpenIR

	Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification
	Zhang Bin 1; Jiang Jiquan 1; Yuan Ying 1; Guan Yifu 1
	2019
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	35 期号:6 页码:1119-1123
摘要	The fluorescence dye SYBR Green II(SG II) has been frequently used in rolling circle amplification(RCA) based analyses of nucleic acids. However, a good amount of inconsistencies have been reported in regards the quality and reproducibility of RCA reactions. To properly examine this experimental issue, here we utilized a series of synthetic oligonucleotides and circular templates to investigate the impact of SG II in RCA reactions. The results indicate that SG II enables a strong fluorescence signal only when complexing with guanosine(G) residue. In RCA reactions, long single-stranded RCA products, enriched with G residues, result in higher fluorescence emission when compared with the addition of other nucleotide residues. These results suggest that the nucleotide composition of the reaction can affect the amplification results and, eventually, can lead to inconsistent fluorescence of the RCA products. This work indicates that particular attention should be given when circular templates are designed for the quantitative analysis of nucleic acids, to further allow the signal reproducibility of RCA-based experiments.
关键词	DNA DETECTION QUADRUPLEX IDENTIFICATION STABILITY Rolling circle amplification SYBR Green II Nucleotide-biased Fluorescence emission
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:6626732
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/155543
专题	中国科学院金属研究所
作者单位	1.中国科学院地质与地球物理研究所 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhang Bin,Jiang Jiquan,Yuan Ying,et al. Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2019,35(6):1119-1123.
APA	Zhang Bin,Jiang Jiquan,Yuan Ying,&Guan Yifu.(2019).Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,35(6),1119-1123.
MLA	Zhang Bin,et al."Influence of Nucleotide-biased Fluorescence Emissions of SYBR Green II on the Result Consistence of Rolling Circle Amplification".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 35.6(2019):1119-1123.