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Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa
Alternative TitleInterleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa
Li FeiYue1; Li Yan2
2009
Source PublicationWORLD JOURNAL OF GASTROENTEROLOGY
ISSN1007-9327
Volume15Issue:29Pages:3621-3630
AbstractAIM: To investigate the correlation between the expression levels of interleukin (IL)-6 and proteins in tight junctions (TJs) in the esophageal mucosa of rats modeling different types of reflux esophagitis (RE), and the ability of aluminum phosphate to protect against RE-induced mucosal damage via these proteins.
Other AbstractAIM: To investigate the correlation between the expression levels of interleukin (IL)-6 and proteins in tight junctions (TJs) in the esophageal mucosa of rats modeling different types of reflux esophagitis (RE), and the ability of aluminum phosphate to protect against RE-induced mucosal damage via these proteins. METHODS: Male SPF Wistar rats aged 56 d were divided randomly into acid RE, alkaline RE, mixed RE, and control groups. Various surgical procedures were performed to establish rat models of acid RE. At 14 d after the procedure, some of the rats started aluminum phosphate treatment. Transmission electron microscopy (TEM) was used to observe the morphological features of TJs and desmosomes in the esophageal epithelium. Immunohistochemical methods and Western blotting were used to measure expression of claudin 1, occludin, ZO-1, JAM-1, DSG-1 and IL-6; reverse transcription polymerase chain reaction (RTPCR) was used to measure expression of mRNA of claudin 1, occludin, ZO-1, JAM-1, DSG-1 and IL-6. RESULTS: At day 14 after the procedures, an RE model was established in all subsequently sacrificed rats of groups A, B and C. By both gross and microscopic observation, the mucosa was damaged and thickened as the disease progressed. With TEM observation, a widened intercellular space was noticed, with significantly fewer desmosomes. Immunohistochemistry showed significantly higher levels of all proteins in all RE models compared to control rats at 3 d after operation (65.5% ± 25.6% vs 20.5% ± 2.1%, P 0.05, treated vs untreated, respectively). These levels increased in the rat with alkaline RE, and this increase was accompanied by continued hyperplasia in comparison with controls (85.5% ± 25.6% vs 20.5% ± 2.1%, P < 0.05, respectively). Furthermore, the expression of TJ proteins was not correlated significantly with that of IL-6 in this group. CONCLUSION: These findings indicate that TJ proteins are highly expressed as an early molecular event involved in RE development, and that IL-6 is an inflammatory factor in this process.
KeywordDILATED INTERCELLULAR SPACES BARRIER FUNCTION OCCLUDIN PERMEABILITY ZO-1 LOCALIZATION INTERACTS DISEASE MODEL Reflux esophagitis Desmosome Tight junction Proteins Mucosa
Indexed ByCSCD
Language英语
Funding Project[China Medical University]
CSCD IDCSCD:3614626
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/155597
Collection中国科学院金属研究所
Affiliation1.Cent Hosp Daqing, Department Digest Dis, Daqing 163000, Heilongjiang Pr, Peoples R China
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Li FeiYue,Li Yan. Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2009,15(29):3621-3630.
APA Li FeiYue,&Li Yan.(2009).Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa.WORLD JOURNAL OF GASTROENTEROLOGY,15(29),3621-3630.
MLA Li FeiYue,et al."Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa".WORLD JOURNAL OF GASTROENTEROLOGY 15.29(2009):3621-3630.
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