Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa

IMR OpenIR

	Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa
其他题名	Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa
	Li FeiYue 1; Li Yan 2
	2009
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	15 期号:29 页码:3621-3630
摘要	AIM: To investigate the correlation between the expression levels of interleukin (IL)-6 and proteins in tight junctions (TJs) in the esophageal mucosa of rats modeling different types of reflux esophagitis (RE), and the ability of aluminum phosphate to protect against RE-induced mucosal damage via these proteins.
其他摘要	AIM: To investigate the correlation between the expression levels of interleukin (IL)-6 and proteins in tight junctions (TJs) in the esophageal mucosa of rats modeling different types of reflux esophagitis (RE), and the ability of aluminum phosphate to protect against RE-induced mucosal damage via these proteins. METHODS: Male SPF Wistar rats aged 56 d were divided randomly into acid RE, alkaline RE, mixed RE, and control groups. Various surgical procedures were performed to establish rat models of acid RE. At 14 d after the procedure, some of the rats started aluminum phosphate treatment. Transmission electron microscopy (TEM) was used to observe the morphological features of TJs and desmosomes in the esophageal epithelium. Immunohistochemical methods and Western blotting were used to measure expression of claudin 1, occludin, ZO-1, JAM-1, DSG-1 and IL-6; reverse transcription polymerase chain reaction (RTPCR) was used to measure expression of mRNA of claudin 1, occludin, ZO-1, JAM-1, DSG-1 and IL-6. RESULTS: At day 14 after the procedures, an RE model was established in all subsequently sacrificed rats of groups A, B and C. By both gross and microscopic observation, the mucosa was damaged and thickened as the disease progressed. With TEM observation, a widened intercellular space was noticed, with significantly fewer desmosomes. Immunohistochemistry showed significantly higher levels of all proteins in all RE models compared to control rats at 3 d after operation (65.5% ± 25.6% vs 20.5% ± 2.1%, P 0.05, treated vs untreated, respectively). These levels increased in the rat with alkaline RE, and this increase was accompanied by continued hyperplasia in comparison with controls (85.5% ± 25.6% vs 20.5% ± 2.1%, P < 0.05, respectively). Furthermore, the expression of TJ proteins was not correlated significantly with that of IL-6 in this group. CONCLUSION: These findings indicate that TJ proteins are highly expressed as an early molecular event involved in RE development, and that IL-6 is an inflammatory factor in this process.
关键词	DILATED INTERCELLULAR SPACES BARRIER FUNCTION OCCLUDIN PERMEABILITY ZO-1 LOCALIZATION INTERACTS DISEASE MODEL Reflux esophagitis Desmosome Tight junction Proteins Mucosa
收录类别	CSCD
语种	英语
资助项目	[China Medical University]
CSCD记录号	CSCD:3614626
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/155597
专题	中国科学院金属研究所
作者单位	1.Cent Hosp Daqing, Department Digest Dis, Daqing 163000, Heilongjiang Pr, Peoples R China 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Li FeiYue,Li Yan. Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2009,15(29):3621-3630.
APA	Li FeiYue,&Li Yan.(2009).Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa.WORLD JOURNAL OF GASTROENTEROLOGY,15(29),3621-3630.
MLA	Li FeiYue,et al."Interleukin-6, desmosome and tight junction protein expression levels in reflux esophagitis-affected mucosa".WORLD JOURNAL OF GASTROENTEROLOGY 15.29(2009):3621-3630.