Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application

doi:10.1016/j.bioactmat.2020.04.019

IMR OpenIR

	Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application
	Gao, Peng; Fan, Bo; Yu, Xiaoming; Liu, Wenwen; Wu, Jie; Shi, Lei; Yang, Di; Tan, Lili; Wan, Peng; Hao, Yulin; Li, Shujun; Hou, Wentao; Yang, Ke; Li, Xiaokang; Guo, Zheng
	2020-09-01
发表期刊	BIOACTIVE MATERIALS
卷号	5 期号:3 页码:680-693
摘要	The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application. Early angiogenesis is important for scaffold survival. It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties. In this study, a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold. For osseointegration and osteogenesis analysis, in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation, adhesion, extracellular matrix (ECM) mineralization and ALP activity compared with bare Ti6Al4V cocultivation. Additionally, MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesis-related genes expression. In vivo studies including fluorochrome labeling, micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation. For angiogenesis studies, magnesium-coated Ti6Al4V improve HUVECs proliferation, adhesion, tube formation, wound-healing and Transwell abilities. HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes (HIF-1 alpha and VEGF) expression. Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation. This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.
DOI	10.1016/j.bioactmat.2020.04.019
WOS记录号	WOS:000546576100023
引用统计	被引频次：100[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/155678
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Gao, Peng,Fan, Bo,Yu, Xiaoming,et al. Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application[J]. BIOACTIVE MATERIALS,2020,5(3):680-693.
APA	Gao, Peng.,Fan, Bo.,Yu, Xiaoming.,Liu, Wenwen.,Wu, Jie.,...&Guo, Zheng.(2020).Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application.BIOACTIVE MATERIALS,5(3),680-693.
MLA	Gao, Peng,et al."Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application".BIOACTIVE MATERIALS 5.3(2020):680-693.