| Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes | |
| Alternative Title | Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes ☆ |
| Wang Yan1; Zhao Fenghong1; Liao Yingjun2; Jin Yaping1; Sun Guifan1 | |
| 2012 | |
| Source Publication | NEURAL REGENERATION RESEARCH
 |
| ISSN | 1673-5374 |
| Volume | 7Issue:31Pages:2439-2445 |
| Abstract | The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 mu M arsenite for 24 hours. Cell viability and morphological observations revealed that 5 mu M arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 mu M arsenite for 24 hours and stimulated with 25 mu M glutamate for 10 minutes. Results showed that Ca2+(i) in astrocytes exposed to 5 and 10 mu M arsenite was significantly increased and levels of D-serine, y-aminobutyric acid and glycine in cultures exposed to 2.5-10 mu M arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 mu M arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function. |
| Other Abstract | The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 μM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 μM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 μM arsenite for 24 hours and stimulated with 25 μM glutamate for 10 minutes. Results showed that Ca 2+ i in astrocytes exposed to 5 and 10 μM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5–10 μM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 μM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function. |
| Keyword | INTELLECTUAL FUNCTION NEURONAL-ACTIVITY DRINKING-WATER GLIAL-CELLS D-SERINE MODULATION RELEVANCE CHILDREN COMMUNICATION BANGLADESH arsenite astrocyte glutamate neuron cell viability intracellular free calcium gliotransmitter neurotoxicity neural regeneration |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [National Natural Science Foundation of China] |
| CSCD ID | CSCD:4694373 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/156051 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.华东师范大学 |
| Recommended Citation GB/T 7714 | Wang Yan,Zhao Fenghong,Liao Yingjun,et al. Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes[J]. NEURAL REGENERATION RESEARCH,2012,7(31):2439-2445. |
| APA | Wang Yan,Zhao Fenghong,Liao Yingjun,Jin Yaping,&Sun Guifan.(2012).Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes.NEURAL REGENERATION RESEARCH,7(31),2439-2445. |
| MLA | Wang Yan,et al."Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes".NEURAL REGENERATION RESEARCH 7.31(2012):2439-2445. |
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