Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes

IMR OpenIR

	Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes
其他题名	Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes ☆
	Wang Yan 1; Zhao Fenghong 1; Liao Yingjun 2; Jin Yaping 1; Sun Guifan 1
	2012
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	7 期号:31 页码:2439-2445
摘要	The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 mu M arsenite for 24 hours. Cell viability and morphological observations revealed that 5 mu M arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 mu M arsenite for 24 hours and stimulated with 25 mu M glutamate for 10 minutes. Results showed that Ca2+(i) in astrocytes exposed to 5 and 10 mu M arsenite was significantly increased and levels of D-serine, y-aminobutyric acid and glycine in cultures exposed to 2.5-10 mu M arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 mu M arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.
其他摘要	The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 μM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 μM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 μM arsenite for 24 hours and stimulated with 25 μM glutamate for 10 minutes. Results showed that Ca 2+ i in astrocytes exposed to 5 and 10 μM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5–10 μM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 μM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.
关键词	INTELLECTUAL FUNCTION NEURONAL-ACTIVITY DRINKING-WATER GLIAL-CELLS D-SERINE MODULATION RELEVANCE CHILDREN COMMUNICATION BANGLADESH arsenite astrocyte glutamate neuron cell viability intracellular free calcium gliotransmitter neurotoxicity neural regeneration
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China]
CSCD记录号	CSCD:4694373
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156051
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.华东师范大学
推荐引用方式 GB/T 7714	Wang Yan,Zhao Fenghong,Liao Yingjun,et al. Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes[J]. NEURAL REGENERATION RESEARCH,2012,7(31):2439-2445.
APA	Wang Yan,Zhao Fenghong,Liao Yingjun,Jin Yaping,&Sun Guifan.(2012).Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes.NEURAL REGENERATION RESEARCH,7(31),2439-2445.
MLA	Wang Yan,et al."Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes".NEURAL REGENERATION RESEARCH 7.31(2012):2439-2445.