Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line

IMR OpenIR

	Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line
其他题名	Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line
	Wang Huihan; Li Yingchun; Liao Aijun; Fu Beibei; Yang Wei; Liu Zhuogang; Wang Xiaobin
	2011
发表期刊	CHINESE JOURNAL OF CANCER RESEARCH
ISSN	1000-9604
卷号	23 期号:1 页码:69-73
摘要	Objective: To observe the reversion of multi-drug resistance by proteasome inhibitor bortezomib in K562/DNR cell line and to analyze the possible mechanism of reversion of multidrug-resistance.
其他摘要	Objective: To observe the reversion of multi‐drug resistance by proteasome inhibitor bortezomib in K562/DNR cell line and to analyze the possible mechanism of reversion of multidrug‐resistance. Methods: MTT method was used to determine the drug resistance of K562/DNR cells and the cellular toxicity of bortezomib. K562/DNR cells were cultured for 12 hours, 24 hours and 36 hours with 100 μg/ml DNR only or plus 4μg/L bortezomib. The expressions of NF‐κB, IκB and P‐gp of K562/DNR were detected with Western blot method, the activity of NF‐κB was tested by ELISA method and the apoptosis rate was observed in each group respectively. Results: The IC50 of DNR on cells of K562/S and K562/DNR groups were 1.16 μg/ml and 50.43 μg/mL, respectively. The drug‐resistant fold was 43.47. The IC10 of PS‐341 on Cell strain K562/DNR was 4 μg/L. Therefore, 4μg/L was selected as the concentration for PS‐341 to reverse drug‐resistance in this study. DNR induced down‐regulation of IκB expression, up‐regulation of NF‐κB and P‐gp expression. After treatment with PS‐341, a proteasome inhibitor, the IκB degradation was inhibited, IκB expression increased, NF‐κB and P‐gp expression decreased in a time dependent manner. Compared to DNR group, the NF‐κB p65 activity of DNR+PS‐341 group was decreased. Compared to Corresponding DNR group, DNR induced apoptosis rate increases after addition of PS‐341 in a time dependent manner. Conclusion: Proteasome inhibitor bortezomib can convert the leukemia cell drug resistance. The mechanism may be that bortezomib decreases the degradation of IκB and the expression of NF‐κB and P‐gp, therefore induces the apoptosis of multi‐drug resistant cells
关键词	MULTIPLE-MYELOMA CELLS FACTOR-KAPPA-B CHEMOTHERAPEUTIC-AGENTS THERAPEUTIC TARGET DRUG-RESISTANCE P-GLYCOPROTEIN LEUKEMIA CANCER MALIGNANCIES SENSITIVITY Bortezomib NF-kappa B Multi-drug resistance mdr1 gene P-gp K562 cells
收录类别	CSCD
语种	英语
资助项目	[Educational Commission of Liaoning Province, China]
CSCD记录号	CSCD:4173900
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156096
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang Huihan,Li Yingchun,Liao Aijun,et al. Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line[J]. CHINESE JOURNAL OF CANCER RESEARCH,2011,23(1):69-73.
APA	Wang Huihan.,Li Yingchun.,Liao Aijun.,Fu Beibei.,Yang Wei.,...&Wang Xiaobin.(2011).Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line.CHINESE JOURNAL OF CANCER RESEARCH,23(1),69-73.
MLA	Wang Huihan,et al."Reversion of Multidrug-Resistance by Proteasome Inhibitor Bortezomib in K562/DNR Cell Line".CHINESE JOURNAL OF CANCER RESEARCH 23.1(2011):69-73.