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Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells
Alternative TitleEffect of activin A on OVCAR-3 Ovarian epithelial cancer cells
Li Xiuqin; Ren Bo; Du Zhenhua
2008
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume20Issue:3Pages:186-189
AbstractObjective: To investigate the proliferation effect and its pathway of activin A on Ovarian epithelial cancer cells line OVCAR-3. Methods: OVCAR-3 cells were cultured in vitro and the membrane receptor ActR II was detected by immunohistochemical method. The OVCAR-3 cells were cultured with 10 ng/ml activin A for 7 d to observe the effects. Activin A at 5, 10, 15 and 20 ng/ml was used separately to treat the OVCAR-3 cancer cells for 24, 48 and 72 h in order to draw the growth proliferation rate curve measured by MTT method. The expression of protein bcl-2 was detected by western-blot. When OVCAR-3 cells were treated with 10 ng/ml activin A and 5 mu g/ml DDP for 24, 48 and 72 h, cell apoptosis could be detected by electron microscopy and flow cytometry (FCM). Results: Positive expression of ActR II was detected. We also found that the proliferation of OVCAR-3 reached to the climax on the 5th day of culture. The experiments showed that the cells treated with activin A increased quickly and grew faster than those in control group. Moreover, OVCAR-3 cells treated with activin A for 48 h proliferated significantly greater than those treated for 24 h or 72 h (P < 0.01). bcl-2 protein expression increased expression in activin A treated group than in control group (P < 0.05). Conclusion: Activin A could increase the proliferation of OVCAR-3 cells which may be through bcl-2 anti-apoptosis pathway.
Other AbstractObjective To investigate the proliferation effect and its pathway of activin A on Ovarian epithelial cancer cells line OVCAR-3. Methods OVCAR-3 cells were cultured in vitro and the membrane receptor ActR II was detected by immunohistochemical method. The OVCAR-3 cells were cultured with 10 ng/ml activin A for 7 d to observe the effects. Activin A at 5, 10, 15 and 20 ng/ml was used separately to treat the OVCAR-3 cancer cells for 24, 48 and 72 h in order to draw the growth proliferation rate curve measured by MTT method. The expression of protein bcl-2 was detected by western-blot. When OVCAR-3 cells were treated with 10 ng/ml activin A and 5 μg/ml DDP for 24, 48 and 72 h, cell apoptosis could be detected by electron microscopy and flow cytometry (FCM).Results Positive expression of ActR II was detected. We also found that the proliferation of OVCAR-3 reached to the climax on the 5th day of culture. The experiments showed that the cells treated with activin A increased quickly and grew faster than those in control group. Moreover, OVCAR-3 cells treated with activin A for 48 h proliferated significantly greater than those treated for 24 h or 72 h (P<0.01). bcl-2 protein expression increased expression in activin A treated group than in control group (P<0.05). Conclusion Activin A could increase the proliferation of OVCAR-3 cells which may be through bcl-2 anti-apoptosis pathway.
KeywordSURFACE EPITHELIUM GROWTH-FACTOR BCL-2 FAMILY EXPRESSION APOPTOSIS INHIBIN Ovarian cancer OVCAR-3 cells line activin A bcl-2 protein MTT
Indexed ByCSCD
Language英语
CSCD IDCSCD:3360139
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/156350
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Li Xiuqin,Ren Bo,Du Zhenhua. Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(3):186-189.
APA Li Xiuqin,Ren Bo,&Du Zhenhua.(2008).Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells.CHINESE JOURNAL OF CANCER RESEARCH,20(3),186-189.
MLA Li Xiuqin,et al."Effect of activin A on OVCAR-3 Ovarian epithelial cancer cells".CHINESE JOURNAL OF CANCER RESEARCH 20.3(2008):186-189.
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