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Mechanism of induction of apoptosis by siRNA targeting hTERT in HeLa cells
Alternative TitleMechanism of induction of apoptosis by siRNA targeting hTERT in HeLa cells
Wang Jian; Ren Changshan
2008
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume20Issue:2Pages:115-120
AbstractObjective: To investigate the molecular mechanism of induction of apoptosis by siRNA targeting human telomerase reverse transcriptase ( hTERT) in HeLa Cells. Methods: HeLa cells were transfected with siRNAs by liposome method. RT-PCR was used to examine mRNA levels of hTERT in HeLa cells. Microarray assay was adopted to explore the transcriptional profiling of apoptosis associated genes. The protein levels of hTERT, TRAIL, Bcl-2, and cytoplasm Cyt c were detected by Western Blotting. The apoptosis rate was determined by flow cytometry using PI staining. Relative activity of Caspase-3 and Caspase-8 was measured by colorimetric assay. Results: The siRNA targeting hTERT suppressed the expression of hTERT gene significantly. Forty-eight hours after transfection, the expression level of TRAIL was increased, the expression level of Bcl-2 was decreased, the releasing of Cyt c was enhanced, the activation of Caspase-3 was increased and the apoptosis rate was increased. Conclusion: hTERT-siRNA induces apoptosis of HeLa cells via activating mitochondrial signal transduction pathway.
Other AbstractObjective:To investigate the molecular mechanism of induction of apoptosis by siRNA targeting human telomerase reverse transcriptase(hTERT)in HeLa Cells.Methods:HeLa cells were transfected with siRNAs by liposome method. RT-PCR was used to examine mRNA levels of hTERT in HeLa cells.Microarray assay was adopted to explore the transcriptional profiling of apoptosis associated genes.The protein levels of hTERT,TRAIL,Bc1-2,and cytoplasm Cyt c were detected by Western Blotting.The apoptosis rate was determined by flow cytometry using PI staining.Relative activity of Caspase-3 and Caspase-8 was measured by colorimetric assay.Results:The siRNA targeting hTERT suppressed the expression of hTERT gene significantly.Forty-eight hours after transfection,the expression level of TRAIL was increased, the expression level of Bcl-2 was decreased,the releasing of Cyt c was enhanced,the activation of Caspase-3 was increased and the apoptosis rate was increased.Conclusion:hTERT-siRNA induces apoptosis of HeLa cells via activating mitochondrial signal transduction pathway.
KeywordPROSTATE-CANCER CELLS INHIBITION EXPRESSION BAX hTERT RNAi apoptosis mitochondrial pathway
Indexed ByCSCD
Language英语
CSCD IDCSCD:3299652
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/156544
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang Jian,Ren Changshan. Mechanism of induction of apoptosis by siRNA targeting hTERT in HeLa cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(2):115-120.
APA Wang Jian,&Ren Changshan.(2008).Mechanism of induction of apoptosis by siRNA targeting hTERT in HeLa cells.CHINESE JOURNAL OF CANCER RESEARCH,20(2),115-120.
MLA Wang Jian,et al."Mechanism of induction of apoptosis by siRNA targeting hTERT in HeLa cells".CHINESE JOURNAL OF CANCER RESEARCH 20.2(2008):115-120.
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