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Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)
Alternative TitleIsolation of hemoglobin with metal–organic frameworks Y(BTC)(H2O)6
Shu Yang1; Meng Ying1; Chen MingLi1; Wang JianHua1
2015
Source PublicationCHINESE CHEMICAL LETTERS
ISSN1001-8417
Volume26Issue:12Pages:1460-1464
AbstractThe hierarchical metal-organic frameworks (MOFs), such as Y(BTC)(H2O)(6), are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature. The product is characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The Y(BTC)(H2O)(6) particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin. The adsorption behavior of hemoglobin onto the Y(BTC)(H2O)(6) fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g(-1). An adsorption efficiency of 87.7% for 100 mu g mL(-1) hemoglobin in 1 mL sample solution (at pH 6.0) is achieved with 0.40 mg Y(BTC)(H2O)(6). 77.3% of the retained hemoglobin is readily recovered using a 0.5% (m/v) SDS solution as the stripping reagent. Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption-desorption process. The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). (C) 2015 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.
Other AbstractThe hierarchical metal–organic frameworks (MOFs), such as Y(BTC)(H_2O)_6, are prepared with yttrium nitrate and benzene-1,3,5-tricarboxylic acid at room temperature. The product is characterized by Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The Y(BTC)(H_2O)_6 particles are sufficiently rigid for performing solid phase extraction and they exhibit favorable selectivity toward the adsorption of hemoglobin. The adsorption behavior of hemoglobin onto the Y(BTC)(H_2O)_6 fits the Langmuir adsorption model with a theoretical adsorption capacity of 555.6 mg g~(-1). An adsorption efficiency of 87.7% for 100 mgmL~(-1) hemoglobin in 1 mL sample solution (at pH 6.0) is achieved with 0.40 mg Y(BTC)(H_2O)_6. 77.3% of the retained hemoglobin is readily recovered using a 0.5% (m/v) SDS solution as the stripping reagent. Circular dichroism spectra indicated that the conformation of hemoglobin is maintained during the adsorption–desorption process. The MOFs material is applied for the isolation of hemoglobin from human blood and the purity of the obtained hemoglobin is further verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE).
KeywordSELECTIVE ISOLATION COMPOSITES ENRICHMENT ADSORPTION PROTEINS Metal-organic frameworks Hemoglobin Solid-phase extraction Protein solation
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Fundamental Research Funds for the Central Universities]
CSCD IDCSCD:5605583
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/156556
Collection中国科学院金属研究所
Affiliation1.东北大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Shu Yang,Meng Ying,Chen MingLi,et al. Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)[J]. CHINESE CHEMICAL LETTERS,2015,26(12):1460-1464.
APA Shu Yang,Meng Ying,Chen MingLi,&Wang JianHua.(2015).Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6).CHINESE CHEMICAL LETTERS,26(12),1460-1464.
MLA Shu Yang,et al."Isolation of hemoglobin with metal-organic frameworks Y(BTC)(H2O)(6)".CHINESE CHEMICAL LETTERS 26.12(2015):1460-1464.
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