Silibinin and indocyanine green-loaded nanoparticles inhibit the growth and metastasis of mammalian breast cancer cells in vitro

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	Silibinin and indocyanine green-loaded nanoparticles inhibit the growth and metastasis of mammalian breast cancer cells in vitro
其他题名	Silibinin and indocyanine green-loaded nanoparticles inhibit the growth and metastasis of mammalian breast cancer cells in vitro
	Sun Huiping 1; Su Jinghan 1; Meng Qingshuo 1; Yin Qi 1; Zhang Zhiwen 1; Yu Haijun 1; Zhang Pengcheng 1; Wang Siling 1; Li Yaping 2
	2016
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
卷号	37 期号:7 页码:941-949
摘要	Aim: To improve the therapeutic efficacy of cancer treatments, combinational therapies based on nanosized drug delivery system (NDDS) has been developed recently. In this study we designed a new NDDS loaded with an anti-metastatic drug silibinin and a photothermal agent indocyanine green (ICG), and investigated its effects on the growth and metastasis of breast cancer cells in vitro.
其他摘要	Aim: To improve the therapeutic efficacy of cancer treatments, combinational therapies based on nanosized drug delivery system (NDDS) has been developed recently. In this study we designed a new NDDS loaded with an anti-metastatic drug silibinin and a photothermal agent indocyanine green (ICG), and investigated its effects on the growth and metastasis of breast cancer cells in vitro . Methods: Silibinin and ICG were self-assembled into PCL lipid nanoparticles (SIPNs). Their physical characteristics including the particle size, zeta potential, morphology and in vitro drug release were examined. 4T1 mammalian breast cancer cells were used to evaluate their cellular internalization, cytotoxicity, and their influences on wound healing, in vitro cell migration and invasion. Results: SIPNs showed a well-defined spherical shape with averaged size of 126.3±0.4 nm and zeta potential of ?10.3±0.2 mV. NIR laser irradiation substantially increased the in vitro release of silibinin from the SIPNs (58.3% at the first 8 h, and 97.8% for the total release). Furthermore, NIR laser irradiation markedly increased the uptake of SIPNs into 4T1 cells. Under the NIR laser irradiation, both SIPNs and IPNs (PCL lipid nanoparticles loaded with ICG alone) caused dose-dependent ablation of 4T1 cells. The wound healing, migration and invasion experiments showed that SIPNs exposed to NIR laser irradiation exhibited dramatic in vitro anti-metastasis effects. Conclusion: SIPNs show temperature-sensitive drug release following NIR laser irradiation, which can inhibit the growth and metastasis of breast cancer cells in vitro .
关键词	COMBINATION THERAPY POLYMERIC MICELLES GOLD NANORODS DOXORUBICIN RELEASE NANOMEDICINE NANOCARRIER PACLITAXEL INVASION DELIVERY breast cancer 4T1 mammalian breast cancer cells metastasis nanoparticles silibinin photothermal therapy NIR laser irradiation
收录类别	CSCD
语种	英语
资助项目	[National Basic Research Program of China] ; [National Natural Science Foundation of China]
CSCD记录号	CSCD:5743041
引用统计	被引频次：3[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156661
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.中国科学院
推荐引用方式 GB/T 7714	Sun Huiping,Su Jinghan,Meng Qingshuo,et al. Silibinin and indocyanine green-loaded nanoparticles inhibit the growth and metastasis of mammalian breast cancer cells in vitro[J]. ACTA PHARMACOLOGICA SINICA,2016,37(7):941-949.
APA	Sun Huiping.,Su Jinghan.,Meng Qingshuo.,Yin Qi.,Zhang Zhiwen.,...&Li Yaping.(2016).Silibinin and indocyanine green-loaded nanoparticles inhibit the growth and metastasis of mammalian breast cancer cells in vitro.ACTA PHARMACOLOGICA SINICA,37(7),941-949.
MLA	Sun Huiping,et al."Silibinin and indocyanine green-loaded nanoparticles inhibit the growth and metastasis of mammalian breast cancer cells in vitro".ACTA PHARMACOLOGICA SINICA 37.7(2016):941-949.