Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells

IMR OpenIR

	Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells
其他题名	Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells ☆
	Zhou Jin; Tian Guoping; Wang Jinge; Luo Xiaoguang; Zhang Siyang; Li Jianping; Li Li; Xu Bing; Zhu Feng; Wang Xia; Jia Chunhong; Zhao Weijin; Zhao Danyang; Xu Aihua
	2012
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	7 期号:34 页码:2689-2697
摘要	This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or A beta(1-40)-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells.
其他摘要	This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation. hUCMSCs were co-cultured with normal or Aβ 1-40 -injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone (no treatment). Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural cells.
关键词	BETA-AMYLOID NEUROTOXICITY ALZHEIMERS-DISEASE CHOLINE-ACETYLTRANSFERASE RAT MODEL IN-VITRO REGENERATIVE MEDICINE HIPPOCAMPAL-NEURONS COGNITIVE FUNCTION SCHWANN-CELLS BONE-MARROW stem cell umbilical cord mesenchymal stem cell co-culture induction differentiation neural cell microtubule-associated protein 2 injured cell Transwell neural regeneration regeneration
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:4747648
引用统计	被引频次：2[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/156952
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Zhou Jin,Tian Guoping,Wang Jinge,et al. Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells[J]. NEURAL REGENERATION RESEARCH,2012,7(34):2689-2697.
APA	Zhou Jin.,Tian Guoping.,Wang Jinge.,Luo Xiaoguang.,Zhang Siyang.,...&Xu Aihua.(2012).Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells.NEURAL REGENERATION RESEARCH,7(34),2689-2697.
MLA	Zhou Jin,et al."Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells".NEURAL REGENERATION RESEARCH 7.34(2012):2689-2697.