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RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid
Alternative TitleRP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid
Li K1; Bi KS1
2006
Source PublicationCHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN1005-9040
Volume22Issue:1Pages:56-60
AbstractA sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase (HPLC) system with a Diamonsil C, column and methanol-acetonitrile-water (8:32:60, volume ratio) ( adjusted to pH = 3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluorophenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0.10 to 25.0 mu g/mL (R-2 = 0.9988, n = 9). The precision was 3.42%-10.10% ; the between-day precision was 2.84%-8.91%; the accuracy was 1.51%-1.26% the mean recovery was 99.9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.
Other AbstractA sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase (HPLC) system with a Diamonsil C18 column and methanol-acetonitfile-water (8: 32: 60, volume ratio) (adjusted to pH = 3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluoro-phenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0. 10 to 25.0μg/mL (R2 = 0. 9988, n = 9). The precision was 3.42%-10. 10%; the between-day precision was 2. 84%-8.91% ; the accuracy was - 1.51%-1.26% ; the mean recovery was 99. 9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.
KeywordDi-Gu-Pi decoction cinnamic acid pharmacokinetics RP-HPLC
Indexed ByCSCD
Language英语
CSCD IDCSCD:2659490
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157133
Collection中国科学院金属研究所
Affiliation1.Beijing University
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Li K,Bi KS. RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(1):56-60.
APA Li K,&Bi KS.(2006).RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,22(1),56-60.
MLA Li K,et al."RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 22.1(2006):56-60.
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