| RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid | |
| Alternative Title | RP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid |
| Li K1; Bi KS1 | |
| 2006 | |
| Source Publication | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| ISSN | 1005-9040 |
| Volume | 22Issue:1Pages:56-60 |
| Abstract | A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase (HPLC) system with a Diamonsil C, column and methanol-acetonitrile-water (8:32:60, volume ratio) ( adjusted to pH = 3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluorophenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0.10 to 25.0 mu g/mL (R-2 = 0.9988, n = 9). The precision was 3.42%-10.10% ; the between-day precision was 2.84%-8.91%; the accuracy was 1.51%-1.26% the mean recovery was 99.9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid. |
| Other Abstract | A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase （HPLC） system with a Diamonsil C18 column and methanol-acetonitfile-water （8： 32： 60, volume ratio） （adjusted to pH = 3.0 with glacial acetic acid） as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-（p-fluoro-phenyl）-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0. 10 to 25.0μg/mL （R2 = 0. 9988, n = 9）. The precision was 3.42%-10. 10%; the between-day precision was 2. 84%-8.91% ; the accuracy was - 1.51%-1.26% ; the mean recovery was 99. 9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid. |
| Keyword | Di-Gu-Pi decoction cinnamic acid pharmacokinetics RP-HPLC |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:2659490 |
| Citation statistics |
Cited Times:2[CSCD]
[CSCD Record]
|
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/157133 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.Beijing University 2.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Li K,Bi KS. RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(1):56-60. |
| APA | Li K,&Bi KS.(2006).RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,22(1),56-60. |
| MLA | Li K,et al."RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 22.1(2006):56-60. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment