RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid

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	RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid
其他题名	RP-HPLC Determination and Pharmacokinetic Comparison of Cinnamic Acid in Rat Plasma After Administration of Di-Gu-Pi Decoction and Pure Cinnamic Acid
	Li K 1; Bi KS 1
	2006
发表期刊	CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
ISSN	1005-9040
卷号	22 期号:1 页码:56-60
摘要	A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase (HPLC) system with a Diamonsil C, column and methanol-acetonitrile-water (8:32:60, volume ratio) ( adjusted to pH = 3.0 with glacial acetic acid) as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-(p-fluorophenyl)-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0.10 to 25.0 mu g/mL (R-2 = 0.9988, n = 9). The precision was 3.42%-10.10% ; the between-day precision was 2.84%-8.91%; the accuracy was 1.51%-1.26% the mean recovery was 99.9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.
其他摘要	A sensitive, simple, and accurate method was developed for the determination and pharmacokinetic comparison of cinnamic acid in rat plasma after the administration of a Traditional Chinese Medicinal preparation, Di-Gu-Pi decoction, and pure cinnamic acid using RP-HPLC. Di-Gu-Pi was extracted with 5% aqueous sodium bicarbonate, which was followed by purification with ion exchange column chromatography. The plasma samples taken from rats were deproteinized with methanol. The reversed-phase （HPLC） system with a Diamonsil C18 column and methanol-acetonitfile-water （8： 32： 60, volume ratio）（adjusted to pH = 3.0 with glacial acetic acid） as the mobile phase was employed for the separation of cinnamic acid in the plasma samples. The detection was set at 272 nm and 3-（p-fluoro-phenyl）-2-propenoic acid was chosen as the internal standard. The calibration curve was linear in a range from 0. 10 to 25.0μg/mL （R2 = 0. 9988, n = 9）. The precision was 3.42%-10. 10%; the between-day precision was 2. 84%-8.91% ; the accuracy was - 1.51%-1.26% ; the mean recovery was 99. 9%. The method was found to be sensitive, simple, accurate and appropriate for the determination of cinnamic acid.
关键词	Di-Gu-Pi decoction cinnamic acid pharmacokinetics RP-HPLC
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:2659490
引用统计	被引频次：2[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157133
专题	中国科学院金属研究所
作者单位	1.Beijing University 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Li K,Bi KS. RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2006,22(1):56-60.
APA	Li K,&Bi KS.(2006).RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,22(1),56-60.
MLA	Li K,et al."RP-HPLC determination and pharmacokinetic comparison of cinnamic acid in rat plasma after administration of Di-Gu-Pi decoction and pure cinnamic acid".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 22.1(2006):56-60.