Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine

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	Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine
其他题名	Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine ☆
	Wang Jun; Ge Jing; Yang Liu; Zhang Haiyan; Li Xuli; Xue Dan
	2012
发表期刊	NEURAL REGENERATION RESEARCH
ISSN	1673-5374
卷号	7 期号:28 页码:2199-2205
摘要	Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-kB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.
其他摘要	Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.
关键词	NMDA RECEPTOR PREECLAMPSIA HYPERHOMOCYSTEINEMIA HYPERTENSION PATHOPHYSIOLOGY MILD JUNCTIONS STRESS DEATH hyperhomocysteinemia homocysteine preeclampsia frontal cortex N-methyl-D-aspartate receptor nerve excitability cell apoptosis pregnancy brain neural regeneration
收录类别	CSCD
语种	英语
资助项目	[General Project of Medical Technology of Military]
CSCD记录号	CSCD:4664136
引用统计	被引频次：1[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157425
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang Jun,Ge Jing,Yang Liu,et al. Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine[J]. NEURAL REGENERATION RESEARCH,2012,7(28):2199-2205.
APA	Wang Jun,Ge Jing,Yang Liu,Zhang Haiyan,Li Xuli,&Xue Dan.(2012).Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine.NEURAL REGENERATION RESEARCH,7(28),2199-2205.
MLA	Wang Jun,et al."Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine".NEURAL REGENERATION RESEARCH 7.28(2012):2199-2205.