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Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine
Alternative TitleBrain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine ☆
Wang Jun; Ge Jing; Yang Liu; Zhang Haiyan; Li Xuli; Xue Dan
2012
Source PublicationNEURAL REGENERATION RESEARCH
ISSN1673-5374
Volume7Issue:28Pages:2199-2205
AbstractHyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-kB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.
Other AbstractHyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats.
KeywordNMDA RECEPTOR PREECLAMPSIA HYPERHOMOCYSTEINEMIA HYPERTENSION PATHOPHYSIOLOGY MILD JUNCTIONS STRESS DEATH hyperhomocysteinemia homocysteine preeclampsia frontal cortex N-methyl-D-aspartate receptor nerve excitability cell apoptosis pregnancy brain neural regeneration
Indexed ByCSCD
Language英语
Funding Project[General Project of Medical Technology of Military]
CSCD IDCSCD:4664136
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157425
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang Jun,Ge Jing,Yang Liu,et al. Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine[J]. NEURAL REGENERATION RESEARCH,2012,7(28):2199-2205.
APA Wang Jun,Ge Jing,Yang Liu,Zhang Haiyan,Li Xuli,&Xue Dan.(2012).Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine.NEURAL REGENERATION RESEARCH,7(28),2199-2205.
MLA Wang Jun,et al."Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine".NEURAL REGENERATION RESEARCH 7.28(2012):2199-2205.
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