| Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine | |
| Alternative Title | Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine ☆ |
| Wang Jun; Ge Jing; Yang Liu; Zhang Haiyan; Li Xuli; Xue Dan | |
| 2012 | |
| Source Publication | NEURAL REGENERATION RESEARCH
 |
| ISSN | 1673-5374 |
| Volume | 7Issue:28Pages:2199-2205 |
| Abstract | Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-kB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats. |
| Other Abstract | Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition, immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex. Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis, increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats. |
| Keyword | NMDA RECEPTOR PREECLAMPSIA HYPERHOMOCYSTEINEMIA HYPERTENSION PATHOPHYSIOLOGY MILD JUNCTIONS STRESS DEATH hyperhomocysteinemia homocysteine preeclampsia frontal cortex N-methyl-D-aspartate receptor nerve excitability cell apoptosis pregnancy brain neural regeneration |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [General Project of Medical Technology of Military] |
| CSCD ID | CSCD:4664136 |
| Citation statistics |
Cited Times:1[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/157425 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Wang Jun,Ge Jing,Yang Liu,et al. Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine[J]. NEURAL REGENERATION RESEARCH,2012,7(28):2199-2205. |
| APA | Wang Jun,Ge Jing,Yang Liu,Zhang Haiyan,Li Xuli,&Xue Dan.(2012).Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine.NEURAL REGENERATION RESEARCH,7(28),2199-2205. |
| MLA | Wang Jun,et al."Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine".NEURAL REGENERATION RESEARCH 7.28(2012):2199-2205. |
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