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Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide
Alternative TitleCooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide
Zhou Hongyang1; Luo Guangcai1; Zhang Zhuan2; Zhou Qiang2; Zhai Xin1; Tang GongLi1
2017
Source PublicationCHINESE JOURNAL OF ORGANIC CHEMISTRY
ISSN0253-2786
Volume37Issue:12Pages:3262-3266
AbstractMicromonospora sp. TP-A0468 harbors multiple biosynthetic gene clusters encoding the biosynthesis of secondary metabolites, including kosinostatin (KST) and rifamycins. In our study on the biosynthesis of KST, a new anthracycline polyketide 554B was isolated from the kstB1 deletion mutant Delta kstB1 and identified as shunt product of KST pathway bearing 4-acetyl-2,3,6-deoxyhexosyl moiety. The unexpected elimination of C-3' hydroxyl suggests a 3,4-dehydratase involved in the biosynthesis of 554B, of which the encoding gene was proposed to located beyond the kst gene cluster. Bioinformatic analysis revealed orf6 of rifamycin biosynthetic gene cluster (rif) was the candidate of required 3,4-dehydratase gene. Deletion of orf6 abolished the production of 554B, confirming the involvement of orf6 in the biosynthesis of 554B. Our study attributed orf6 to the origin of 2,3,6-deoxyhexosyl and unveiled the cooperation between biosynthetic gene clusters in Micromonospora sp. TP-A0468.
KeywordANTITUMOR-ACTIVITY KOSINOSTATIN anthracycline hexose 3,4-dehydratase biosynthesis gene cluster cooperation
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Science and Technology Comission Shanghai Municipality]
CSCD IDCSCD:6146551
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157456
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.中国科学院
Recommended Citation
GB/T 7714
Zhou Hongyang,Luo Guangcai,Zhang Zhuan,et al. Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide[J]. CHINESE JOURNAL OF ORGANIC CHEMISTRY,2017,37(12):3262-3266.
APA Zhou Hongyang,Luo Guangcai,Zhang Zhuan,Zhou Qiang,Zhai Xin,&Tang GongLi.(2017).Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide.CHINESE JOURNAL OF ORGANIC CHEMISTRY,37(12),3262-3266.
MLA Zhou Hongyang,et al."Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide".CHINESE JOURNAL OF ORGANIC CHEMISTRY 37.12(2017):3262-3266.
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