Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide

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	Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide
其他题名	Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide
	Zhou Hongyang 1; Luo Guangcai 1; Zhang Zhuan 2; Zhou Qiang 2; Zhai Xin 1; Tang GongLi 1
	2017
发表期刊	CHINESE JOURNAL OF ORGANIC CHEMISTRY
ISSN	0253-2786
卷号	37 期号:12 页码:3262-3266
摘要	Micromonospora sp. TP-A0468 harbors multiple biosynthetic gene clusters encoding the biosynthesis of secondary metabolites, including kosinostatin (KST) and rifamycins. In our study on the biosynthesis of KST, a new anthracycline polyketide 554B was isolated from the kstB1 deletion mutant Delta kstB1 and identified as shunt product of KST pathway bearing 4-acetyl-2,3,6-deoxyhexosyl moiety. The unexpected elimination of C-3' hydroxyl suggests a 3,4-dehydratase involved in the biosynthesis of 554B, of which the encoding gene was proposed to located beyond the kst gene cluster. Bioinformatic analysis revealed orf6 of rifamycin biosynthetic gene cluster (rif) was the candidate of required 3,4-dehydratase gene. Deletion of orf6 abolished the production of 554B, confirming the involvement of orf6 in the biosynthesis of 554B. Our study attributed orf6 to the origin of 2,3,6-deoxyhexosyl and unveiled the cooperation between biosynthetic gene clusters in Micromonospora sp. TP-A0468.
关键词	ANTITUMOR-ACTIVITY KOSINOSTATIN anthracycline hexose 3,4-dehydratase biosynthesis gene cluster cooperation
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Science and Technology Comission Shanghai Municipality]
CSCD记录号	CSCD:6146551
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157456
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.中国科学院
推荐引用方式 GB/T 7714	Zhou Hongyang,Luo Guangcai,Zhang Zhuan,et al. Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide[J]. CHINESE JOURNAL OF ORGANIC CHEMISTRY,2017,37(12):3262-3266.
APA	Zhou Hongyang,Luo Guangcai,Zhang Zhuan,Zhou Qiang,Zhai Xin,&Tang GongLi.(2017).Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide.CHINESE JOURNAL OF ORGANIC CHEMISTRY,37(12),3262-3266.
MLA	Zhou Hongyang,et al."Cooperation of Two Biosynthetic Gene Clusters in the Biosynthesis of New Anthracycline Polyketide".CHINESE JOURNAL OF ORGANIC CHEMISTRY 37.12(2017):3262-3266.