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ZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016
Alternative TitleZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016
Fu Shihong1; Song Song1; Liu Hong4; Li Yuanyuan1; Li Xiaolong1; Gao Xiaoyan1; Xu Ziqian1; Liu Guoping6; Wang Dingming7; Tian Zhenzao7; Zhou Jingzhu7; He Ying1; Lei Wenwen1; Wang Huanyu1; Wang Bin3; Lu Xiaoqing3; Liang Guodong1
2017
Source PublicationSCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
Volume60Issue:12Pages:1364-1371
AbstractA field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells (C6/36) and mammalian cells (BHK-21) for virus isolation. The results indicated that 40% (3,540/8,795) were Anopheles sinensis, 30% (2,700/8,795) were Culex pipiens quinquefasciatus, and 29% (2,530/8,795) were Armigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses (ZIKVs) isolated from Culex pipiens quinquefasciatus and Armigeres subbalbeatus, respectively; three Japanese encephalitis viruses (JEVs) isolated from Culex pipiens quinquefasciatus; two Banna viruses (BAVs) isolated from Culex pipiens quinquefasciatus and Anopheles sinensis, respectively; and one densovirus (DNV) isolated from Culex pipiens quinquefasciatus. The ZIKVs isolated from the Culex pipiens quinquefasciatus and Armigeres subbalbeatus mosquitoes represent the first ZIKV isolates in mainland China. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue.
Other AbstractA field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells (C6/36) and mammalian cells (BHK-21) for virus isolation. The results indicated that 40% (3,540/8,795) were Anopheles sinensis, 30% (2,700/8,795) were Culex pipiens quinquefasciatus, and 29% (2,530/8,795) were Armigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses (ZIKVs) isolated from Culex pipiens quinquefasciatus and Armigeres subbalbeatus, respectively; three Japanese encephalitis viruses (JEVs) isolated from Culex pipiens quinquefasciatus; two Banna viruses (BAVs) isolated from Culex pipiens quinquefasciatus and Anopheles sinensis, respectively; and one densovirus (DNV) isolated from Culex pipiens quinquefasciatus. The ZIKVs isolated from the Culex pipiens quinquefasciatus and Armigeres subbalbeatus mosquitoes represent the first ZIKV isolates in mainland China. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue.
KeywordJAPANESE-ENCEPHALITIS GUIZHOU PROVINCE EVOLUTION ARBOVIRUSES INFECTION EMERGENCE SPREAD FUTURE arbovirus surveillance mosquito-borne arbovirus Japanese encephalitis virus Zika virus
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Special National Project on Research and Development of Key Biosafety Technologies] ; [National Key Plan for Scientific Research and Development of China] ; [Development Grants of State Key Laboratory of Infectious Disease Prevention and Control] ; [National Key Research and Development Program of China] ; [Open Research Fund Program of Wuhan National Bio-Safety Level 4 Lab of CAS]
CSCD IDCSCD:6150336
Citation statistics
Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157488
Collection中国科学院金属研究所
Affiliation1.Chinese Ctr Dis Control & Prevent, Natl Institute Viral Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Beijing 102206, Peoples R China Natl Institute Viral Dis Control & Prevent State Key Lab Infect Dis Prevent & Control
2.Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China
3.青岛大学
4.山东大学
5.Chinese Ctr Dis Control & Prevent, Natl Institute Parasit Dis, WHO Collaborating Ctr Trop Dieases, Key Lab Parasite & Vector Biol,Minist Hlth, Shanghai 200025, Peoples R China Natl Institute Parasit Dis WHO Collaborating Ctr Trop Dieases Key Lab Parasite & Vector Biol,Minist Hlth
6.中国科学院金属研究所
7.Guizhou Prov Ctr Dis Control & Prevent, Guiyang 550004, Guizhou, Peoples R China
Recommended Citation
GB/T 7714
Fu Shihong,Song Song,Liu Hong,et al. ZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016[J]. SCIENCE CHINA-LIFE SCIENCES,2017,60(12):1364-1371.
APA Fu Shihong.,Song Song.,Liu Hong.,Li Yuanyuan.,Li Xiaolong.,...&Liang Guodong.(2017).ZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016.SCIENCE CHINA-LIFE SCIENCES,60(12),1364-1371.
MLA Fu Shihong,et al."ZIKA virus isolated from mosquitoes: a field and laboratory investigation in China, 2016".SCIENCE CHINA-LIFE SCIENCES 60.12(2017):1364-1371.
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