IMR OpenIR
Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration
Alternative TitleTransient expression of strictosidine synthase in tobacco leaves by vacuum infiltration
Wang M1; Li QR1
2002
Source PublicationACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN0582-9879
Volume34Issue:6Pages:703-706
AbstractStrictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay.
Other AbstractStrictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay.
KeywordCATHARANTHUS-ROSEUS TRYPTOPHAN DECARBOXYLASE NICOTIANA-TABACUM CELL-CULTURES SEQUENCE GENES strictosidine synthase vacuum infiltration subcellular compartments Nicotiana tabacum
Indexed ByCSCD
Language英语
CSCD IDCSCD:1141013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157679
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Wang M,Li QR. Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(6):703-706.
APA Wang M,&Li QR.(2002).Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(6),703-706.
MLA Wang M,et al."Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.6(2002):703-706.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang M]'s Articles
[Li QR]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang M]'s Articles
[Li QR]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang M]'s Articles
[Li QR]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.