| Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration | |
| Alternative Title | Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration |
| Wang M1; Li QR1 | |
| 2002 | |
| Source Publication | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| ISSN | 0582-9879 |
| Volume | 34Issue:6Pages:703-706 |
| Abstract | Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay. |
| Other Abstract | Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay. |
| Keyword | CATHARANTHUS-ROSEUS TRYPTOPHAN DECARBOXYLASE NICOTIANA-TABACUM CELL-CULTURES SEQUENCE GENES strictosidine synthase vacuum infiltration subcellular compartments Nicotiana tabacum |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:1141013 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/157679 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院 2.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Wang M,Li QR. Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(6):703-706. |
| APA | Wang M,&Li QR.(2002).Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(6),703-706. |
| MLA | Wang M,et al."Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.6(2002):703-706. |
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