Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration

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	Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration
其他题名	Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration
	Wang M 1; Li QR 1
	2002
发表期刊	ACTA BIOCHIMICA ET BIOPHYSICA SINICA
ISSN	0582-9879
卷号	34 期号:6 页码:703-706
摘要	Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay.
其他摘要	Strictosidine synthase (STR) is the key enzyme involved in early steps of biosynthesis of monoterpenoid indole alkaloids. STR catalyzes the condensation of tryptamine with secologanin into strictosidine. The gene encoding STR targeted to different subcellular compartments was transiently expressed in the tobacco leaves. In vitro STR enzymatic activity was measured by the depletion of tryptamine indicated by fluorescence. The results showed that the recombinant STR was effectively expressed as soluble protein in three subcellular compartments - chloroplast, vacuole and endoplasmic reticulum in the leaves of tobacco by Western blot analysis and STR enzymatic assay.
关键词	CATHARANTHUS-ROSEUS TRYPTOPHAN DECARBOXYLASE NICOTIANA-TABACUM CELL-CULTURES SEQUENCE GENES strictosidine synthase vacuum infiltration subcellular compartments Nicotiana tabacum
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:1141013
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157679
专题	中国科学院金属研究所
作者单位	1.中国科学院 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Wang M,Li QR. Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2002,34(6):703-706.
APA	Wang M,&Li QR.(2002).Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,34(6),703-706.
MLA	Wang M,et al."Transient expression of strictosidine synthase in tobacco leaves by vacuum infiltration".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 34.6(2002):703-706.