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Fungal diversity in major oil-shale mines in China
Alternative TitleFungal diversity in major oil-shale mines in China
Jiang Shaoyan1; Wang Wenxing1; Xue Xiangxin2; Cao Chengyou1; Zhang Ying1
2016
Source PublicationJOURNAL OF ENVIRONMENTAL SCIENCES
ISSN1001-0742
Volume41Pages:81-89
AbstractAs an insufficiently utilized energy resource, oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins. However, little is known about fungal diversity in oil shale. Polymerase chain reaction cloning was used to construct the fungal ribosomal deoxyribonucleic acid internal transcribed spacer (rDNA ITS) clone libraries of Huadian Mine in Jilin Province, Maoming Mine in Guangdong Province, and Fushun Mine in Liaoning Province. Pure culture and molecular identification were applied for the isolation of cultivable fungi in fresh oil shale of each mine. Results of clone libraries indicated that each mine had over 50% Ascomycota (58.4%-98.9%) and 1.1%-13.5% unidentified fungi. Fushun Mine and Huadian Mine had 5.9% and 28.1% Basidiomycota, respectively. Huadian Mine showed the highest fungal diversity, followed by Fushun Mine and Maoming Mine. Jaccard indexes showed that the similarities between any two of three fungal communities at the genus level were very low, indicating that fungi in each mine developed independently during the long geological adaptation and formed a community composition fitting the environment. In the fresh oil-shale samples of the three mines, cultivable fungal phyla were consistent with the results of clone libraries. Fifteen genera and several unidentified fungi were identified as Ascomycota and Basidiomycota using pure culture. Penicillium was the only genus found in all three mines. These findings contributed to gaining a clear understanding of current fungal resources in major oil-shale mines in China and provided useful information for relevant studies on isolation of indigenous fungi carrying functional genes from oil shale. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
Other AbstractAs an insufficiently utilized energy resource, oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins. However, little is known about fungal diversity in oil shale. Polymerase chain reaction cloning was used to construct the fungal ribosomal deoxyribonucleic acid internal transcribed spacer (rDNA ITS) clone libraries of Huadian Mine in Jilin Province, Maoming Mine in Guangdong Province, and Fushun Mine in Liaoning Province. Pure culture and molecular identification were applied for the isolation of cultivable fungi in fresh oil shale of each mine. Results of clone libraries indicated that each mine had over 50% Ascomycota (58.4%–98.9%) and 1.1%–13.5% unidentified fungi. Fushun Mine and Huadian Mine had 5.9% and 28.1% Basidiomycota, respectively. Huadian Mine showed the highest fungal diversity, followed by Fushun Mine and Maoming Mine. Jaccard indexes showed that the similarities between any two of three fungal communities at the genus level were very low, indicating that fungi in each mine developed independently during the long geological adaptation and formed a community composition fitting the environment. In the fresh oil-shale samples of the three mines, cultivable fungal phyla were consistent with the results of clone libraries. Fifteen genera and several unidentified fungi were identified as Ascomycota and Basidiomycota using pure culture. Penicillium was the only genus found in all three mines. These findings contributed to gaining a clear understanding of current fungal resources in major oil-shale mines in China and provided useful information for relevant studies on isolation of indigenous fungi carrying functional genes from oil shale.
KeywordORGANIC-MATTER MICROBIAL COMMUNITIES BLACK SHALES DEGRADATION BIODEGRADATION SOILS Oil shale Fungal diversity Clone library rDNA ITS Pure culture
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Fundamental Research Funds for the Central Universities] ; [China Postdoctoral Science Foundation]
CSCD IDCSCD:5666354
Citation statistics
Cited Times:2[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157682
Collection中国科学院金属研究所
Affiliation1.东北大学
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Jiang Shaoyan,Wang Wenxing,Xue Xiangxin,et al. Fungal diversity in major oil-shale mines in China[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2016,41:81-89.
APA Jiang Shaoyan,Wang Wenxing,Xue Xiangxin,Cao Chengyou,&Zhang Ying.(2016).Fungal diversity in major oil-shale mines in China.JOURNAL OF ENVIRONMENTAL SCIENCES,41,81-89.
MLA Jiang Shaoyan,et al."Fungal diversity in major oil-shale mines in China".JOURNAL OF ENVIRONMENTAL SCIENCES 41(2016):81-89.
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