Fungal diversity in major oil-shale mines in China

IMR OpenIR

	Fungal diversity in major oil-shale mines in China
其他题名	Fungal diversity in major oil-shale mines in China
	Jiang Shaoyan 1; Wang Wenxing 1; Xue Xiangxin 2; Cao Chengyou 1; Zhang Ying 1
	2016
发表期刊	JOURNAL OF ENVIRONMENTAL SCIENCES
ISSN	1001-0742
卷号	41 页码:81-89
摘要	As an insufficiently utilized energy resource, oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins. However, little is known about fungal diversity in oil shale. Polymerase chain reaction cloning was used to construct the fungal ribosomal deoxyribonucleic acid internal transcribed spacer (rDNA ITS) clone libraries of Huadian Mine in Jilin Province, Maoming Mine in Guangdong Province, and Fushun Mine in Liaoning Province. Pure culture and molecular identification were applied for the isolation of cultivable fungi in fresh oil shale of each mine. Results of clone libraries indicated that each mine had over 50% Ascomycota (58.4%-98.9%) and 1.1%-13.5% unidentified fungi. Fushun Mine and Huadian Mine had 5.9% and 28.1% Basidiomycota, respectively. Huadian Mine showed the highest fungal diversity, followed by Fushun Mine and Maoming Mine. Jaccard indexes showed that the similarities between any two of three fungal communities at the genus level were very low, indicating that fungi in each mine developed independently during the long geological adaptation and formed a community composition fitting the environment. In the fresh oil-shale samples of the three mines, cultivable fungal phyla were consistent with the results of clone libraries. Fifteen genera and several unidentified fungi were identified as Ascomycota and Basidiomycota using pure culture. Penicillium was the only genus found in all three mines. These findings contributed to gaining a clear understanding of current fungal resources in major oil-shale mines in China and provided useful information for relevant studies on isolation of indigenous fungi carrying functional genes from oil shale. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
其他摘要	As an insufficiently utilized energy resource, oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins. However, little is known about fungal diversity in oil shale. Polymerase chain reaction cloning was used to construct the fungal ribosomal deoxyribonucleic acid internal transcribed spacer (rDNA ITS) clone libraries of Huadian Mine in Jilin Province, Maoming Mine in Guangdong Province, and Fushun Mine in Liaoning Province. Pure culture and molecular identification were applied for the isolation of cultivable fungi in fresh oil shale of each mine. Results of clone libraries indicated that each mine had over 50% Ascomycota (58.4%–98.9%) and 1.1%–13.5% unidentified fungi. Fushun Mine and Huadian Mine had 5.9% and 28.1% Basidiomycota, respectively. Huadian Mine showed the highest fungal diversity, followed by Fushun Mine and Maoming Mine. Jaccard indexes showed that the similarities between any two of three fungal communities at the genus level were very low, indicating that fungi in each mine developed independently during the long geological adaptation and formed a community composition fitting the environment. In the fresh oil-shale samples of the three mines, cultivable fungal phyla were consistent with the results of clone libraries. Fifteen genera and several unidentified fungi were identified as Ascomycota and Basidiomycota using pure culture. Penicillium was the only genus found in all three mines. These findings contributed to gaining a clear understanding of current fungal resources in major oil-shale mines in China and provided useful information for relevant studies on isolation of indigenous fungi carrying functional genes from oil shale.
关键词	ORGANIC-MATTER MICROBIAL COMMUNITIES BLACK SHALES DEGRADATION BIODEGRADATION SOILS Oil shale Fungal diversity Clone library rDNA ITS Pure culture
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Fundamental Research Funds for the Central Universities] ; [China Postdoctoral Science Foundation]
CSCD记录号	CSCD:5666354
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157682
专题	中国科学院金属研究所
作者单位	1.东北大学 2.中国科学院金属研究所
推荐引用方式 GB/T 7714	Jiang Shaoyan,Wang Wenxing,Xue Xiangxin,et al. Fungal diversity in major oil-shale mines in China[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2016,41:81-89.
APA	Jiang Shaoyan,Wang Wenxing,Xue Xiangxin,Cao Chengyou,&Zhang Ying.(2016).Fungal diversity in major oil-shale mines in China.JOURNAL OF ENVIRONMENTAL SCIENCES,41,81-89.
MLA	Jiang Shaoyan,et al."Fungal diversity in major oil-shale mines in China".JOURNAL OF ENVIRONMENTAL SCIENCES 41(2016):81-89.