Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats

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	Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats
其他题名	Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats
	Sun YingJie; Chen WeiMin; Zhang TieZheng; Cao HuiJuan; Zhou Jin
	2008
发表期刊	WORLD JOURNAL OF GASTROENTEROLOGY
ISSN	1007-9327
卷号	14 期号:38 页码:5868-5875
摘要	AIM: To investigate the tight junction protein expressions of intestinal mucosa in an experimental model of cardiopulmonary bypass (CPB) in rats.
其他摘要	AIM: To investigate the tight junction protein expressions of intestinal mucosa in an experimental model of cardiopulmonary bypass (CPB) in rats. METHODS: Thirty anesthetized rats were randomly divided into two groups: Group S (n=10) served as sham operation and group C (n = 20) served as CPB which underwent CPB for 1 h. Expression of occludin and zonula occludens-1 (ZO-1) were determined by Western blotting and immunotochemistry, respectively. Plasma levels of diamine oxidase (DAO) and d-lactate were determined using an enzymatic spectrophotometry.RESULTS: Immunohistochemical localization of occludin and ZO-1 showed disruption of the tight junctions in enterocytes lining villi at the end of CPB and 2 h after CPB. The intensities of the occludin and ZO-1 at the end of CPB were lower than those of control group (76.4%±22.5% vs 96.5%±28.5% and 62.4%±10.1% vs 85.5%±25.6%, P <0.05) and were further lower at 2 h after CPB (50.5% ±10.5% and 45.3%±9.5%, P <0.05). Plasma d-lactate and DAO levels increased significantly (8.688 ±0.704 vs 5.745±0.364 and 0.898±0.062 vs 0.562±0.035, P<0.05) at the end of CPB compared with control group and were significantly higher at 2 h after CPB than those at the end of CPB (9.377 ±0.769 and 1.038±0.252, P <0.05). There were significant negative correlations between occludin or ZO-1 expression and DAO (r2= 0.5629,r2= 0.5424, P <0.05) or d-lactate levels (r2 = 0.6512,r2 = 0.7073, P <0.05) both at the end of CPB and 2 h after CPB.CONCLUSION: CPB markedly down-regulates the expression of occludin and ZO-1 proteins in intestinal mucosa of rats. The close correlation between expression of tight junctions (TJs) and plasma levels of DAO or d-lactate supports the hypothesis that intestinal permeability increases during and after CPB because of decreases in the expressions of TJs.
关键词	INDUCED INFLAMMATORY RESPONSE MULTIPLE ORGAN FAILURE BLOOD LACTATE LEVELS HUMAN SEPTIC SHOCK NITRIC-OXIDE BACTERIAL TRANSLOCATION EPITHELIAL BARRIER DIAMINE OXIDASE CARDIAC-SURGERY PERMEABILITY Cardiopulmonary bypass Occludin ZO-1 Tight junction Diamine oxidase d-lactate
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3431832
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157747
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Sun YingJie,Chen WeiMin,Zhang TieZheng,et al. Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2008,14(38):5868-5875.
APA	Sun YingJie,Chen WeiMin,Zhang TieZheng,Cao HuiJuan,&Zhou Jin.(2008).Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats.WORLD JOURNAL OF GASTROENTEROLOGY,14(38),5868-5875.
MLA	Sun YingJie,et al."Effects of cardiopulmonary bypass on tight junction protein expressions in intestinal mucosa of rats".WORLD JOURNAL OF GASTROENTEROLOGY 14.38(2008):5868-5875.