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MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice
Alternative TitleMULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice
Yu Haiping1; Ren Deyong2; Zhu Yangzhou1; Xu Jiangmin1; Wang Yuexing2; Liu Ruifang2; Fang Yunxia2; Shi Zhenyuan2; Pan Jiangjie2; Lu Mei1; Ma Bojun1; Hu Jiang2; Rao Yuchun1
2016
Source PublicationSCIENCE BULLETIN
ISSN2095-9273
Volume61Issue:23Pages:1810-1817
AbstractPlant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650 (BC12) in mtd1 mutant had a single nucleotide substitution (G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant. Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, alpha-amylase activity analysis and gibberellic acid (GA) treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.
Other AbstractPlant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650 (BC12) in mtd1 mutant had a single nucleotide substitution (G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant. Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, a-amylase activity analysis and gibberellic acid (GA) treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.
KeywordGENETIC-ANALYSIS MUTANT-GENE DWARF BIOSYNTHESIS DEGRADATION ENCODES PROTEIN IDENTIFICATION ARCHITECTURE OUTGROWTH Rice Dwarf Tillering mtd1 Gene cloning
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Zhejiang Provincial Natural Science Foundation of China] ; [China Postdoctoral Science Foundation] ; [Open Foundation from Zhejiang Provincial Top Key Discipline of Biology]
CSCD IDCSCD:5886791
Citation statistics
Cited Times:1[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157827
Collection中国科学院金属研究所
Affiliation1.浙江师范大学
2.China Natl Rice Res Institute, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China
3.中国科学院金属研究所
Recommended Citation
GB/T 7714
Yu Haiping,Ren Deyong,Zhu Yangzhou,et al. MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice[J]. SCIENCE BULLETIN,2016,61(23):1810-1817.
APA Yu Haiping.,Ren Deyong.,Zhu Yangzhou.,Xu Jiangmin.,Wang Yuexing.,...&Rao Yuchun.(2016).MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice.SCIENCE BULLETIN,61(23),1810-1817.
MLA Yu Haiping,et al."MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice".SCIENCE BULLETIN 61.23(2016):1810-1817.
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