MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice

IMR OpenIR

	MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice
其他题名	MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice
	Yu Haiping 1; Ren Deyong 2; Zhu Yangzhou 1; Xu Jiangmin 1; Wang Yuexing 2; Liu Ruifang 2; Fang Yunxia 2; Shi Zhenyuan 2; Pan Jiangjie 2; Lu Mei 1; Ma Bojun 1; Hu Jiang 2; Rao Yuchun 1
	2016
发表期刊	SCIENCE BULLETIN
ISSN	2095-9273
卷号	61 期号:23 页码:1810-1817
摘要	Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650 (BC12) in mtd1 mutant had a single nucleotide substitution (G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant. Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, alpha-amylase activity analysis and gibberellic acid (GA) treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.
其他摘要	Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650 (BC12) in mtd1 mutant had a single nucleotide substitution (G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant. Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, a-amylase activity analysis and gibberellic acid (GA) treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.
关键词	GENETIC-ANALYSIS MUTANT-GENE DWARF BIOSYNTHESIS DEGRADATION ENCODES PROTEIN IDENTIFICATION ARCHITECTURE OUTGROWTH Rice Dwarf Tillering mtd1 Gene cloning
收录类别	CSCD
语种	英语
资助项目	[National Natural Science Foundation of China] ; [Zhejiang Provincial Natural Science Foundation of China] ; [China Postdoctoral Science Foundation] ; [Open Foundation from Zhejiang Provincial Top Key Discipline of Biology]
CSCD记录号	CSCD:5886791
引用统计	被引频次：6[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157827
专题	中国科学院金属研究所
作者单位	1.浙江师范大学 2.China Natl Rice Res Institute, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China 3.中国科学院金属研究所
推荐引用方式 GB/T 7714	Yu Haiping,Ren Deyong,Zhu Yangzhou,et al. MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice[J]. SCIENCE BULLETIN,2016,61(23):1810-1817.
APA	Yu Haiping.,Ren Deyong.,Zhu Yangzhou.,Xu Jiangmin.,Wang Yuexing.,...&Rao Yuchun.(2016).MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice.SCIENCE BULLETIN,61(23),1810-1817.
MLA	Yu Haiping,et al."MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice".SCIENCE BULLETIN 61.23(2016):1810-1817.